产品介绍 | | |
| Mouse antiListeria monocytogenesantibody, clone LZF7recognizes both intactListeria spand outer membrane preparations reacting with a ~37 kDa protein in western blotting. Mouse antiListeria monocytogenesantibody, clone LZF7 recognizes strains ofListeria monocytogenes.Mouse antiListeria monocytogenesantibody, clone LZF7 has also been shown to cross-react with otherListeriaspecies includingL. innocua,L. ivanoviiandL. welshimeri. |
产品详情 | | |
Target Species | Bacterial |
Product Form | Purified IgG - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein G from ascites |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | <0.1% Sodium Azide (NaN3) |
Immunogen | Listeria monocytogenes, outer membrane fraction. |
Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | | |
WesternBlotting | √ | | |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.- ELISA
- This product is suitable for use as a detection antibody in a sandwich ELISA, with0400-0030as the coating antibody. Please note, the activity of this antibody is reduced on boiling of the bacteria.
- Western Blotting
- 0400-0027 detects a band of approximately 23kDa in reduced blots of cell fragments.
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文献 |
| 1.Marusov, G.et al.(2012) A microarray biosensor for multiplexed detection of microbes using grating-coupled surface plasmon resonance imaging.Environ Sci Technol. 46 (1): 348-59.2.Veerapandian, M. and Neethirajan, S. (2015) Graphene oxide chemically decorated with Ag-Ru/chitosan nanoparticles: Fabrication, Electrode Processing and Immunosensing PropertyRSC Adv. Aug 21 [Epub ahead of print] |
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