Recombinant Protein G:HRP
货号：740605
规格：1 ml
价格：2406
产品类型：蛋白
品牌：Bio-Rad
产品介绍 | | |
| Recombinant Protein Gis a preparation of streptococcal protein G expressed inE. coli. Protein G binds to the Fc portion of immunoglobulin G subclasses (IgGs) from a broad range of species including human and mouse, and does not bind IgA, IgE, IgM or serum albumin. |
产品详情 | | |
Target Species | Bacterial |
Product Form | Recombinant protein - conjugated to Horseradish Peroxidase (HRP) - liquid |
Preparation | Recombinant protein G fromStreptococcusstrain G148, expressed inE.coli. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | 50% Glycerol |
Protein Molecular Weight | 22 kDa (monomeric protein lacking cystine residues) |
Regulatory | For research purposes only |
Guarantee | Guaranteed until date of expiry. Please see product label. |
存储条件 | | |
| Store at -20oC only.Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
| ELISA | √ | 1/4000 | 1/8000 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. |
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文献 |
| 1.Saegerman et al (2004) Evalution of three serum i-ELISAs using monoclonal antibodies and protein G as peroxidase conjugate for the diagnosis of bovine brucellosis.Vet Microbiol. 20. 91-105.2.Qi et al (2001) Chromatography on DEAE ion-exchange and Protein G affinity columns in tandem for the separation and purification of proteins.Biochem Biophys Methods. 49. 263-273.3.McDonald, J.U.et al.(2016) Development of a custom pentaplex sandwich immunoassay using Protein-G coupled beads for the Luminex® xMAP® platform.J Immunol Methods. pii: S0022-1759(16)30033-3. [Epub ahead of print] |
技术参数 产品应用 ELISA
产品类型 Recombinant Protein
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