| 产品介绍 | | |
| Goat anti Human collagen IV antibodyrecognizes human collagen type IV. It has been cross adsorbed against collagen types I, II, III, V and VI, resulting in <10% cross-reactivity. It may cross-react with collagen type IV from other species.Collagen is located in the extracellular matrix of connective tissues. It is part of the interacting network of proteoglycans and proteins that provides a structural framework for both soft and calcified connective tissues. Collagen Type IV forms a two-dimensional reticulum and is a major component of the basal lamina.Goat anti human collagen IV has been successfully employed for the demonstration of vasculature in developing rat pups using immunofluorescence techniques (Kermorvant-Ducheminet al.2013). |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified IgG - liquid |
Antiserum Preparation | Antiserum to human collagen IV was raised by repeated immunisation of goats with highly purified antigen.Purified IgG was prepared from whole serum by affinity chromatography. |
Buffer Solution | Borate buffered saline |
Preservative Stabilisers | None present |
Approx. Protein Concentrations | IgG concentration 0.4 mg/ml |
Immunogen | Human Collagen Type IV from human placenta |
Regulatory | For research purposes only |
Guarantee | Guaranteed until date of expiry. Please see product label. |
| 存储条件 | | |
| Store at -20℃only.Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | 1/1000 | 1/4000 |
Immunoblotting | √ | | |
| Immunofluorescence | √ | | |
Immunohistology - Frozen | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. |
| 二抗相关产品 |
Description | Product Code | Applications | Pack Size |
Rabbit anti Goat IgG (Fc):FITC | STAR122F | Flow | 1 mg |
Rabbit anti Goat IgG (Fc):HRP | STAR122P | C;E;WB | 1 mg |
数据 |
Published customer image:Goat anti Human collagen IV antibody used (134001) for the demonstration of rat retinal vasculature by immunofluorescence.Image caption:Figure 4. Astrocytes and pericytes phenotype in NHIR. P1 rat pups were injected with low doses of streptozotocin (STZ) or with the citrate buffer vehicle (CTL). A–D. Retinal flatmounts and sections of P6 CTL (A–B) and STZ (C–D) were co-stained using anti-collagen IV antibody and anti-GFAP antibody. E–F Retinal flatmounts of P6 CTL (E) and STZ (F) were co-stained using anti-collagen IV antibody and or anti-Ng2 antibody. G–H. STZ animals with hyperglycemia >400 mg/DL were co-stained using anti-collagen IV antibody and anti-Ng2 antibody. Nuclei were counterstained with DAPI in A and C. Scale bar 50 μm in A–G, g and g′; 5 μm in E and F insets and H.From: Kermorvant-Duchemin E, Pinel AC, Lavalette S, Lenne D, Raoul W, Calippe B, et al. (2013) Neonatal Hyperglycemia Inhibits Angiogenesis and Induces Inflammation and Neuronal Degeneration in the Retina.PLoS ONE 8(11): e79545.This image is from an open access article distributed under terms of aCreative Commons Attribution License. |
| 文献 |
| 1. Kermorvant-Duchemin, E.et al.(2013) Neonatal hyperglycemia inhibits angiogenesis and induces inflammation and neuronal degeneration in the retina. PLoS One. 8(11): e79545.2. Dominguez, E.et al.(2015) Experimental Branch Retinal Vein Occlusion Induces Upstream Pericyte Loss and Vascular Destabilization. PLoS One. 10 (7): e0132644.3. Rajapaksa, K.S.et al.(2016) Preclinical Safety Profile of a Depleting Antibody against CRTh2 for Asthma: Well Tolerated Despite Unexpected CRTh2 Expression on Vascular Pericytes in the Central Nervous System and Gastric Mucosa. Toxicol Sci. 152 (1): 72-84.4. Trouillet, A.et al.(2017) Col4a1 mutation generates vascular abnormalities correlated with neuronal damage in a mouse model of HANAC syndrome. Neurobiol Dis. 100: 52-61.5. Roubeix, C.et al.(2019) Mo-derived perivascular macrophage recruitment protects against endothelial cell death in retinal vein occlusion. J Neuroinflammation. 16 (1): 157. |
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