CD73 Monoclonal Antibody (7G2)

货号：41-0200

规格：100 µg

价格：4809

产品类型：流式抗体

品牌：Thermo Fisher

物种：人

宿主：小鼠

抗体亚型：IgG2a, kappa

克隆号： 7G2

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.5 mg/mL	用法：	1 µg/mL (WB)；Assay-dependent (IHC (F))；1:100(ICC/IF)；Assay-dependent (Flow)

靶标信息CD73 (Ecto-5-prime-nucleotidase, 5-prime-ribonucleotide phosphohydrolase) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. CD73 consists of a dimer of 2 identical 70 kDa subunits bound externally to the plasma membrane by a glycosyl phosphatidyl inositol linkage. CD73 is used as a marker of lymphocyte differentiation. Consequently, a deficiency of CD73 occurs in a variety of immunodeficiency diseases. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from CD73 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. The CD73 gene has been localized to 6q14-q21 by immunofluorescence and a study of a panel of human x mouse hybrids that contained fragments of chromosome 6 as translocations. Defects in the CD73 gene can lead to the calcification of joints and arteries, and intestinal tuberculosis. Two transcript variants encoding different isoforms of CD73 have been found.

数据

CD73 Antibody (41-0200) in ICC/IFImmunofluorescence analysis of CD73 was performed using 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CD73 Monoclonal Antibody (7G2) (Product # 41-0200) at a concentration of 1 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300 dilution). Panel d represents the merged image showing membrane localization. Panel e represents HeLa cells showing no expression of CD73. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

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参考文献：

1.Cytometry. Part A : the journal of the International Society for Analytical CytologyMesenchymal markers on human adipose stem/progenitor cells."41-0200 was used in immunohistochemistry to identify the mesenchymal markers on human adipose stem/progenitor cells"2.Tissue antigensProduction and characterization of monoclonal antibodies to the glycosyl phosphatidylinositol-anchored lymphocyte differentiation antigen ecto-5'-nucleotidase (CD73).AuthorsThomson LF,Ruedi JM,Glass A,Moldenhauer G,Moller P,Low MG,Klemens MR,Massaia M,Lucas AH3.Biological researchExpression regulation and functional analysis of RGS2 and RGS4 in adipogenic and osteogenic differentiation of human mesenchymal stem cells."41-0200 was used in Immunocytochemistry to show that regulators of G protein signalling proteins may play multifaceted supportive or conflicting roles during human adipogenesis and osteogenesis."4.Stem cell reviews and reportsCharacterization of Tunneling Nanotubes in Wharton's jelly Mesenchymal Stem Cells. An Intercellular Exchange of Components between Neighboring Cells."410200 was used in immunocytochemistry to describe the types, structure, components, dynamics and functionality of the tunneling nanotubes bridging neighboring human umbilical cord mesenchymal stem cells obtained from Wharton's jelly"5.Journal of anatomyMorphological and immunocytochemical characteristics indicate the yield of early progenitors and represent a quality control for human mesenchymal stem cell culturing.

技术参数

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