SB590885
货号：04-0080
规格：2 mg
价格：0
产品类型：小分子化合物
品牌：Stemgent
The triarylimidazole derivative Stemoleculetm SB590885 is a potent (KI,app<0.2 nM) for and selective (>10-fold selectivity against C-RAF) inhibitor of B-RAF kinase.1 SB590885 blocks MAPK activation, proliferation, transformation, and tumorigenicity of B-RAF-expressing malignant cells. SB590885 has been shown to support the conversion and maintenance of human naïve stem cells.2
Stemgent conveniently provides all the components comprising the 5i/L/A media supplment necessary to support naive pluripotent stem cell applications.
5i (inhibitors):
PD0325901 (04-0006),
Y27632 (04-0012),
WH-4-023 (04-0079),
SB590885 (04-0080),
IM-12 (04-0081) or alternatively,
CHIR99021 (04-0004)
L: LIF, Human Recombinant (03-0016), and
A: Activin A, Human Recombinant (03-0001)
产品详情
| Product Name | StemoleculetmSB590885 |
| Catalog Number | 04-0080 |
| Size | 2 mg |
| Alternate Name | 5-(2-(4-(2-Dimethylaminoethoxy)phenyl)-5-(4-pyridinyl)-1H-imidazol-4-yl)-2,3-dihydro-1H-inden-1-one oxime |
| Chemical Formula | C27H27N6O2 |
| Molecular Weight | 453.54 |
| CAS Number | 405554-55-4 |
| Purity | >99% by HPLC analysis |
| Appearance | Light yellow to yellow color solid |
| Solubility | SB590885 is soluble in DMSO at 100mL and in ethanol at 1mM at room temperature. For a 10mM concentrated stock solution of SB590885 in DMSO, reconstitute the compound by adding 441 uL of DMSO to the entire contents of the vial. If precipitate is observed, warm the solution to 37○C for 2 to 5 minutes. For cell culture, the media should be pre-warmed prior to adding the reconstituted compound. The maximum concentration recommended for dilution in media to ensure complete solubility is 10uM. Note: for most cells, the maximum tolerance to DMSO is less than 0.5% by volume. |
| Storage and Stability | Store powder for up to six months at 4○C, protected from light. Following reconstitution, store aliquots at -20○C. |
| Quality Control | The purity of SB590885 was determined by HPLC analysis. The accurate mass was determined by mass spectrometry. Cellular toxicity of SB590885 was tested on mouse embryonic stem cells. |
参考文献
King AJ, Patrick DR, Batorsky RS, Ho ML, Do HT, Zhang SY, Kumar R, Rusnak DW, Takle AK, Wilson DM, Hugger E, Wang L, Karreth F, Lougheed JC, Lee J, Chau D, Stout TJ, May EW, Rominger CM, Schaber MD, Luo L, Lakdawala AS, Adams JL, Contractor RG, Smalley KS, Herlyn M, Morrissey MM, Tuveson DA, Huang PS. “Demonstration of a genetic therapeutic index for tumors expressing oncogenic BRAF by the kinase inhibitor SB-590885.” Cancer Res. 66(23):11100-5 (2006).
Theunissen TW, Powell BE, Wang H, Mitalipova M, Faddah DA, Reddy J, Fan ZP, Maetzel D, Ganz K, Shi L, Lungjangwa T, Imsoonthornruksa S, Stelzer Y, Rangarajan S, D’Alessio A, Zhang J, Gao Q, Dawlaty MM, Young RA, Gray NS, Jaenisch R. “Systematic identification of culture conditions for induction and maintenance of naïve human pluripotency.” Cell Stem Cell 15(4):471-87 (2014).
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