StainAlive™ Stemgent® StainAlive™ TRA-1-81 Antibody (DyLight™ 488), Mouse anti-Human
货号：09-0069
规格：100 μl
价格：0
产品类型：流式抗体
品牌：Stemgent
物种：人
宿主：小鼠
抗体亚型：IgM
克隆号：TRA-1-81
荧光染料：Purified
The TRA-1-81 monoclonal antibody reacts with a pluripotent-stem-cell-specific antigen expressed upon the surface of human embryonic stem (ES) cells, embryonal carcinoma (EC) cells, and embryonic germ (EG) cells. The expression of TRA-1-81 antigen is stage-specific; as human EC and ES cells undergo differentiation, expression of TRA-1-81 antigen is lost, making it a widely used marker to characterize human ES cells and to monitor their differentiation. TRA-1-81 antibody recognizes a carbohydrate epitope expressed on podocalyxin, a sialoglycoprotein structurally related to CD34. Podocalyxin is a transmembrane glycoprotein which has been implicated in the development of aggressiveness in a variety of cancers, including breast and prostate cancer. For use on live cells, this antibody has been specifically formulated with low endotoxin and no sodium azide. ES cell morphology, proliferation, and expression of pluripotency markers does not change after staining with this antibody.
产品详情
| Size | 100 μl |
| Concentration | 0.5 mg/ml |
| Clone | TRA-1-81 |
| Isotype | Mouse IgM, κ |
| Immunogen | Human embryonal carcinoma cell line 2102Ep |
| Reactivity | Human |
| Preparation | This antibody was purified by affinity chromatography and conjugated with DyLight 488 under optimal conditions. The solution is free of unconjugated dye and unlabeled antibody. 0.2 μm filter sterilized. Formulation is free from bacteria, fungi, mycoplasma, and mouse virus. |
| Formulation | Phosphate-buffered solution, pH 7.2, containing no preservative |
| Storage and Stability | Store at 4°C protected from light. Do not freeze. Handle under aseptic conditions. Stable for 6 months from date of receipt when stored as directed. |
| Quality Control | Tested by immunocytochemistry to ensure product quality. |
| Endotoxin level is less than 0.1 EU/μg (0.01 ng/μg) of the protein as determined by the LAL assay. |
| Recommended Usage | The suggested use of this antibody is a 1:100 dilution for immunocytochemistry. |
数据
ICC analysis of TRA-1-81 antibody on primary human iPS cell colonies generated by reprogramming with mRNA. Phase contrast view of cells in culture and the same field of view after staining with StainAlive TRA-1-81 Antibody (DyLight 488) using a 1:100 dilution. [A] Phase contrast view of cells in culture, [B] same field of view after staining with StainAlive TRA-1-81 Antibody (DyLight 488) using a 1:100 dilution.
参考文献
1. Andrews, P.W., Banting, G., Damjanov, I., Arnaud, D., and Avner, P. (1984) Three monoclonal antibodies defining distinct differentiation antigens associated with different high molecular weight polypeptides on the surface of human embryonal carcinoma cells. Hybridoma 3: 347-361.
2. Yu, J., Vodyanik, M.A., Smuga-Otto, K., Antosiewicz-Bourget, J., Frane, J.L., Tian, S., Nie, J., Jonsdottir, G.A., Ruotti, V., Stewart, R., Slukvin, I.I., and Thomson, J.A. (2007) Induced pluripotent stem cell lines derived from human somatic cells. Science 318: 1917-1920.
3. Henderson, J.K., Draper, J.S., Baillie, H.S., Fishel, S., Thomson, J.A., Moore, H., and Andrews, P.W. (2002) Preimplantation human embryos and embryonic stem cells show comparable expression of stage-specific embryonic antigens. Stem Cells 20: 329-337.
4. Schopperle, W.M., and DeWolf, W.C. (2007) The TRA-1-60 and TRA-1-81 human pluripotent stem cell markers are expressed on podocalyxin in embryonal carcinoma. Stem Cells 25: 723-730.
5. Poleganov MA; Eminli S; Beissert T; Herz S; Moon J-I; Goldmann J; Beyer A; Heck R; Burkhart I; Roldan DB; Tureci O; Yi K; Hamilton B; Sahin U. "Efficient reprogramming of human fibroblasts and blood-derived endothelial progenitor cells using nonmodified RNA for reprogramming and immune evasion." Human Gene Therapy 26:751 (2015)
6. Haraguchi Y; Matsuura K; Shimizu T; Yamato M; Okano T. "Simple suspension culture system of human iPS cells maintianting the pluripotency for cardiac cell sheet engineering." J Tissue Eng Regen Medicine 9:1363 (2015)
7. Mack, A., Kroboth, S., Rajesh, D., Wang, W. B. Generation of Induced Pluripotent Stem Cells from CD34+ Cells across Blood Drawn from Multiple Donors with Non-Integrating Episomal Vectors. PLoS One; 6(11): e27956.(2011)
©
Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：
https://www.biorod.cn/thread-14937.htm
QQ:1749072012