CD4 Monoclonal Antibody (GK1.5), FITC, eBioscience™

货号：11-0041-81,11-0041-82,11-0041-85,11-0041-86

规格：50 µg,100 µg,500 µg,1 mg

价格：633,831,1129,4509

产品类型：流式抗体

品牌：eBioscience

抗原：CD4

物种：人/小鼠/大鼠

宿主：大鼠

抗体亚型：IgG2b, kappa

克隆号：GK1.5

荧光染料：FITC

抗体类型:	流式抗体	同型对照：	Rat IgG2b kappa Isotype Control (eB149/10H5), FITC, eBioscience™
浓度：	0.5 mg/mL	用法：	0.25µg/test(Flow）

产品详细信息Description: The GK1.5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of GK1.5 is blocked by RM4-5.Applications Reported: The GK1.5 antibody has been reported for use in flow cytometric analysis.Applications Tested: This GK1.5 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.25 µg per test A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered. 靶标信息The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

数据

CD4 Antibody (11-0041-82) in FlowStaining of mouse thymocytes with Anti-Mouse CD8a PE (Product # 12-0081-82) (left)r mouse splenocytes with Anti-Mouse CD3e PE (Product # 12-0031-82) (right) andAnti-Mouse CD4 FITC. Total viable cells were used for analysis. CD4 Antibody (11-0041-82)Staining of mouse splenocytes. As expected based on known relative expression patterns,CD4 clone GK1.5 stains about 25% of total lymphocytes and does not stain CD45R (B220)+ B cells.Details: Balb/c splenocytes were surface stained with CD45R (B220, clone RA3-6B2) and Rat IgG2bIsotype Control or CD4 (clone GK1.5). Cells in the lymphocyte gate were used for analysis. Relativeexpression validation info.Antibody target was verified by Relative expression to ensure the antibody binds to the antigen stated.

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参考文献：

1.Frontiers in pharmacologyTraditional Herbal Medicine-Derived Sulforaphene LFS-01 Reverses Colitis in Mice by Selectively Altering the Gut Microbiota and Promoting Intestinal Gamma-Delta T Cells.2.Oxidative medicine and cellular longevityGenetic Nrf2 Overactivation Inhibits the Deleterious Effects Induced by Hepatocyte-Specific c-met Deletion during the Progression of NASH.3.Nature communicationsIntratumoral modulation of the inducible co-stimulator ICOS by recombinant oncolytic virus promotes systemic anti-tumour immunity.4.Journal of immunology (Baltimore, Md. : 1950)Multispectral Imaging of T and B Cells in Murine Spleen and Tumor.5.Infection and immunityDistinct Contributions of CD4+ and CD8+ T Cells to Pathogenesis of Trypanosoma brucei Infection in the Context of Gamma Interferon and Interleukin-10.

技术参数

产品应用 Flow；FN；IF；ICC；IHC；IHC (P)；WB；IHC (F)

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