CD357 (AITR/GITR) Monoclonal Antibody (DTA-1), APC

货号：17-5874-81

规格：50μg

价格：2112

产品类型：流式抗体

品牌：Thermo Fisher

物种：小鼠

宿主：大鼠

抗体亚型：IgG2b, kappa

克隆号：DTA-1

荧光染料：APC

抗体类型:	单抗	同型对照：	Rat IgG2b kappa Isotype Control, APC, eBioscience™
免疫原性：		用法：	0.06 µg/test(Flow）

Description: The DTA-1 monoclonal antibody reacts with mouse GITR, Glucocorticoid-Induced TNFR family gene, also known as TNFRSF18. In naive mice, GITR is expressed predominantly by CD4+CD25+ T regulatory cells (Treg) and by CD25+ CD4+ CD8- thymocytes. Stimulation of GITR with DTA-1 antibody abrogates Treg cell-mediated suppression. Although it does not deplete CD357 expressing cells in vivo, it has been shown to induce tumorogenesis. The removal of GITR-expressing Treg cells or the administration of DTA-1 resulted in organ specific autoimmune disease.Applications Reported: This DTA-1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This DTA-1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationGlucocorticoid-induced tumor necrosis factor receptor family-related gene (GITR) is a member of the tumor necrosis factor receptor (TNFR) family that is expressed at low levels on unstimulated T cells, B cells, and macrophages. Upon activation, CD4+ and CD8+ T cells up-regulate GITR expression, whereas immunoregulatory T cells constitutively express high levels of GITR.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD357 (AITR/GITR) Antibody (17-5874-81) in Flow Staining of C57BL/6 splenocytes with Anti-Mouse CD25 PE (Product # 12-0251-82) and 0.03 µg of Rat IgG2b K Isotype Control APC (Product # 17-4031-82) (left) or 0.03 µg of Anti-Mouse CD357 (GITR) APC (right).Total viable cells were used for analysis.

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参考文献：

1. PloS oneOptimal isolation of functional Foxp3+ induced regulatory T cells using DEREG mice."17-5874 was used in Flow cytometry/Cell sorting to define culture conditions for efficient generation of eGFP(+)Foxp3(+) iTregs by use of DEREG mice."AuthorsBaru AM,Untucht C,Ganesh V,Hesse C,Mayer CT,Sparwasser T2. Journal of immunology (Baltimore, Md. : 1950)Chronic follicular bronchiolitis requires antigen-specific regulatory T cell control to prevent fatal disease progression."17-5874 was used in Flow cytometry/Cell sorting to create and characterize a new model of autoimmune lung inflammation that targets the medium and small airways."AuthorsSchmitt EG,Haribhai D,Jeschke JC,Co DO,Ziegelbauer J,Yan K,Iwakura Y,Mishra MK,Simpson P,Salzman NH,Williams CB3. Human gene therapyCytokine-conditioned dendritic cells induce humoral tolerance to protein therapy in mice."17-5874 was used in Flow cytometry/Cell sorting to demonstrate that autologous cell therapy for antigen-targeted immune suppression may be developed to facilitate long-term therapy."AuthorsSule G,Suzuki M,Guse K,Cela R,Rodgers JR,Lee B4. PloS onec-Rel controls multiple discrete steps in the thymic development of Foxp3+ CD4 regulatory T cells."17-5874 was used in Flow cytometry/Cell sorting to show that c-Rel generates a permissive state for foxp3 transcription during the development of nTreg precursors."AuthorsGrigoriadis G,Vasanthakumar A,Banerjee A,Grumont R,Overall S,Gleeson P,Shannon F,Gerondakis S5. Neuro-oncologyThymus-derived rather than tumor-induced regulatory T cells predominate in brain tumors."17-5874 was used in Flow cytometry/Cell sorting to investigate whether brain-tumour-resident Tregs are thymus-derived natural Tregs or induced Tregs, showing that thymus-derived Tregs predominate."AuthorsWainwright DA,Sengupta S,Han Y,Lesniak MS

技术参数

产品应用 Flow

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