BrdU Monoclonal Antibody (BU20A), APC

货号：17-5071-42,17-5071-41

规格：100 Tests,23 Tests

价格：3371,1311

产品类型：流式抗体

品牌：eBioscience

物种：人/小鼠

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：BU20A

荧光染料：APC

抗体类型:	单抗	同型对照：	Mouse IgG1 kappa Isotype Control, APC, eBioscience™
免疫原性：		用法：	5uL（0.125µg）/test(Flow）

Description: This Bu20a monoclonal antibody reacts with 5-bromodeoxyuridine (BrdU). BrdU is a derivative of uridine that can be incorporated into DNA in place of thymidine during the S-phase of the cell cycle. Anti-BrdU can then be used to identify cells that have undergone DNA synthesis during BrdU treatment. For staining for flow cytometric analysis, we recommend the use of the BrdU Staining Buffer Set (cat. 00-5525) and protocol.Applications Reported: This BU20A antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This BU20A antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of BrdU-labeled mouse splenocytes using the Foxp3/Transcription Factor Buffer Set (cat. 00-5523) and protocol or the BrdU Staining Buffer Set (cat. 00-5525) and protocol. Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.BrdU labeling and staining with the Anti-BrdU antibody:1. Label dividing cells with 10 µM BrdU for 45 min at 37°C.2. Following the incubation, harvest the cells and wash once with 1X PBS.3. Stain surface molecules according to the Surface Staining Protocol.4. Wash in cold Flow Cytometry Staining Buffer or 1X PBS.5. Resuspend the cell pellet by pulse vortexing. Then add 1 mL of freshly prepared Foxp3 Fixation/Permeabilization Buffer (cat. 00-5521) to each sample. pulse vortex again.6. Incubate for 30 to 60 minutes at 2-8°C in the dark.7. Wash once with cold Flow Cytometry Staining Buffer followed by centrifugation. Decant the supernatant.8. Resuspend the cell pellet with 100 µL Flow Cytometry Staining Buffer containing 30 µg of Dnase I.9. Incubate for 1 hr at 37°C and then wash.10. Stain cells with anti-BrdU antibody for 30 min to 1 hr and then wash.10. Analyze the samples.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationBromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues, and can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

BrdU Antibody (17-5071-42) in Flow Anti-CD3/CD28 (Product # 16-0031-82, 16-0281)-stimulated mouse splenocytes either unlabeled (left) or labeled with BrdU (right) were surface stained with Anti-Mouse CD4 PE (Product # 12-0041-82). These cells were thenstained intracellularly with Anti-BrdU APC using the BrdU Staining Kit for Flow Cytometry APC and protocol.Total viable cells were used for analysis.

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参考文献：

1. The Journal of clinical investigationFOXP1 controls mesenchymal stem cell commitment and senescence during skeletal aging.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2. PloS oneProgrammed death 1 regulates memory phenotype CD4 T cell accumulation, inhibits expansion of the effector memory phenotype subset and modulates production of effector cytokines."17-5071 was used in Flow cytometry/Cell sorting to demonstrate that PD-1 has an important role in determining the composition and functional aspects of the memory phenotype CD4 T cell pool."AuthorsCharlton JJ,Tsoukatou D,Mamalaki C,Chatzidakis I3. Nature cell biologyBreast-cancer-secreted miR-122 reprograms glucose metabolism in premetastatic niche to promote metastasis.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer4. Molecular biology of the cellCENP-32 is required to maintain centrosomal dominance in bipolar spindle assembly.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer5. eLifeFunctional genomic screening reveals asparagine dependence as a metabolic vulnerability in sarcoma.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer

技术参数

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