CD40 Monoclonal Antibody (1C10), Functional Grade, eBioscience

货号：16-0401-81,16-0401-82,16-0401-85,16-0401-86

规格：50μg,100μg,500μg,1mg

价格：967,1930,3389,6633

产品类型：流式抗体

品牌：eBioscience

抗原：CD40

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：1C10

荧光染料：其它

抗体类型:	功能抗体	同型对照：	Rat IgG2a kappa Isotype Control, Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	1 μg/test(Flow)；Assay-Dependent (FN)

Description: The 1C10 monoclonal antibody reacts with mouse CD40, a 45-50 kDa type I transmembrane glycoprotein. CD40 is a member of the TNFR family and is expressed by mouse B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells. CD40 regulates B cell development/maturation by inducing Ig isotype switching and in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with CD154 (gp39), its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation.The monoclonal antibody 1C10 is reported to have agonistic activity in vitro and in vivo.Applications Reported: The 1C10 antibody has been reported for use in flow cytometric analysis. It has also been reported in in vitro activation of B cells.Applications Tested: The 1C10 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.Background/Target InformationThe CD40 antigen is a single chain glycoprotein that is known to be a member of the tumor necrosis factor/nerve growth factor superfamily and shows a significant homology to the Hodgkin's disease associated antigen, CD30. CD40 is present on all B cells except plasma cells and is also found on some epithelial cells, carcinomas and lymphoid dendritic cells. CD40 has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of CD40, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with CD40 and serves as a mediator of the signal transduction. The interaction of CD40 and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Two alternatively spliced transcript variants of CD40 encoding distinct isoforms have been reported. Diseases associated with CD40 dysfunction include Type 3 Hyper-Igm immunodeficiency and CD40 ligand deficiency.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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参考文献：

1. Frontiers in immunologyFcεRI γ-Chain Negatively Modulates Dectin-1 Responses in Dendritic Cells.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2. EBioMedicineDendritic Cells Aggravates Atherosclerosis via Th1 Cell Induction and Enhanced CCL5 Expression in Plaque Macrophages.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer3. Molecular medicine reportsDiscrimination of the heterogeneity of bone marrow‑derived dendritic cells.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer4. PloS oneMyD88/CD40 Genetic Adjuvant Function in Cutaneous Atypical Antigen-Presenting Cells Contributes to DNA Vaccine Immunogenicity.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer5. OncotargetN-glycosylation converts non-glycoproteins into mannose receptor ligands and reveals antigen-specific T cell responses in vivo."16-0401 was used in Flow cytometry/Cell sorting to analyse whether enforced N-glycosylation is a suited strategy to enhance the immunogenicity of non-glycosylated antigens for vaccination studies."AuthorsKreer C,Kuepper JM,Zehner M,Quast T,Kolanus W,Schumak B,Burgdorf S

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