Chicken anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 647/鸡抗羊 IgG (H+L)交叉吸附， Alexa Fluor 647

货号：A-21469

规格：500μl

价格：3410

产品类型：荧光二抗

品牌：Thermo Fisher

物种：山羊

宿主：其它

抗体亚型：IgG

荧光染料：Alexa Fluor 647

点击查看所有Alexa Fluor 荧光二抗

抗体类型:	荧光二抗	同型对照：	IgG
免疫原性：	Gamma Immunoglobins Heavy and Light chains	用法：	1-10 μg/mL（IHC）；1-10 μg/mL (ICC)；1-10 μg/mL (IF)

This Chicken anti-goat antibody reacts with IgG heavy chains and all classes of immunoglobulin light chains from goat. Chicken secondary antibodies have gained popularity because they demonstrate a lower level of nonspecific binding. Chicken antibodies lack a classic “Fc” domain and will not bind to protein A or protein G, nor will they bind to mammalian IgG Fc receptors. Fluorescence of this long-wavelength Alexa Fluor dye is not visible by looking through a conventional fluorescence microscope.Background/Target InformationAnti-Goat secondary antibodies are affinity-purified antibodies with well-characterized specificity for goat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

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参考文献：

1. PloS oneCD34 Promotes Pathological Epi-Retinal Neovascularization in a Mouse Model of Oxygen-Induced Retinopathy."A21469 was used in flow cytometry to determine the function of CD34 in tip cells and angiogenesis"AuthorsSiemerink MJ,Hughes MR,Dallinga MG,Gora T,Cait J,Vogels IM,Yetin-Arik B,Van Noorden CJ,Klaassen I,McNagny KM,Schlingemann RO2. eLifeAXL receptor tyrosine kinase is required for T cell priming and antiviral immunity."A21469 was used in flow cytometry to use knock out mice to establish the role of AXL during viral infection"AuthorsSchmid ET,Pang IK,Carrera Silva EA,Bosurgi L,Miner JJ,Diamond MS,Iwasaki A,Rothlin CV3. Nature communicationsInduced p53 loss in mouse luminal cells causes clonal expansion and development of mammary tumours."A21469 was used in immunohistochemistry - paraffin section to show that induction p53 loss in Krt8(+) mammary luminal cells leads to their clonal expansion without directly affecting their luminal identity"AuthorsTao L,Xiang D,Xie Y,Bronson RT,Li Z4. Journal of immunology (Baltimore, Md. : 1950)NK cells regulate CD8+ T cell priming and dendritic cell migration during influenza A infection by IFN-γ and perforin-dependent mechanisms.AuthorsGe MQ,Ho AW,Tang Y,Wong KH,Chua BY,Gasser S,Kemeny DM5. Antimicrobial agents and chemotherapyRole of cathepsin A and lysosomes in the intracellular activation of novel antipapillomavirus agent GS-9191.AuthorsBirkus G,Kutty N,Frey CR,Shribata R,Chou T,Wagner C,McDermott M,Cihlar T

技术参数

产品应用 ICC;IF;IHC

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