Cyclophilin 40 Polyclonal Antibody/亲环素D内参抗体

货号： PA3-022

规格：100 µL

价格：5441

产品类型：内参抗体

品牌：Thermo Fisher

物种：人/小鼠/大鼠

宿主：兔

抗体亚型：其它

荧光染料：其它

点击Invitrogen内参抗体集，查看更多内参抗体

抗体类型:	多抗	同型对照：	IgG
免疫原性：	Synthetic peptide corresponding to residues C A(356) Q K D K E K A V Y A K M F A(370) of human Cyp-40.	用法：	1:100（IF）；1:200（IHC(F)）1:1000(WB)

Product Specific InformationPA3-022 detect cyclophilin 40 (CyP-40) from human and rat and mouse tissues and cells. This antibody does not cross-react with CyPA.PA3-022 has been successfully used in Western blot, iF, immunohistochemistry, immunocytochemistry, immunoprecipitation, gel shift and FACS procedures. By Western blot, this antibody detects a 40 kDa protein representing CyP-40 from rat brain extract.The PA3-022 immunogen is a synthetic peptide corresponding to residues C A(356) Q K D K E K A V Y A K M F A(370) of human Cyp-40.Background/Target InformationImmunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents: cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP).Both CyP:CsA and FKBP:FK506 complexes have been shown to inhibit calcineurin, a calcium and calmodulin dependent protein phosphatase which has been implicated as an important signaling enzyme in T-cell activation, providing a possible mechanism of immunosuppression by CsA and FK506. Immunophilins function as peptidyl prolyl cis-trans-isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. As PPIase's, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline containing proteins.CyP-40, a 40 kDa protein, shares significant homology with smaller CyPA (CyP-18) and FKBP59. CyP-40 exhibits the characteristic CsA binding and isomerase activity of CyP-18, though these activities appear to be less with CyP-40 than with Cyp-18. Like FKBP59, CyP-40 has been found in progesterone receptor complexes. CyP-40 is expressed at similar levels in many tissues.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

Cyclophilin 40 Antibody (PA3-022) in WBWestern blot of Cyp 40 on rat spleen extracts using Product # PA3-022. Cyclophilin 40 Antibody (PA3-022) in IFImmunofluorescent analysis of Cyclophilin D in A431 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Cyclophilin D polyclonal antibody (Product # PA3-022) at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35552). Cyclophilin D staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification. Cyclophilin 40 Antibody (PA3-022) in IHCImmunohistochemistry was performed on normal deparaffinized human Skeletalmuscle tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a rabbit polyclonal antibody recognizing Cyclophilin D (Product # PA3-022) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

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参考文献：

1.BMC neuroscienceA mitochondrial division inhibitor, Mdivi-1, inhibits mitochondrial fragmentation and attenuates kainic acid-induced hippocampal cell death."PA3-022 was used in western blot to study inhibition of mitochondrial fragmentation and attenuation of kainic acid-induced hippocampal cell death by a mitochondrial division inhibitor, Mdivi-1"AuthorsKim H,Lee JY,Park KJ,Kim WH,Roh GS2.Molecular and cellular biologyThe physical association of multiple molecular chaperone proteins with mutant p53 is altered by geldanamycin, an hsp90-binding agent."PA3-022 was used in immunohistochemistry to investigate the mechanisms for the aberrant association of chaperones and mutant p53 and the intracellular accumulation of mutant p53."AuthorsWhitesell L,Sutphin PD,Pulcini EJ,Martinez JD,Cook PH3.CellRepression of heat shock transcription factor HSF1 activation by HSP90 (HSP90 complex) that forms a stress-sensitive complex with HSF1."PA3-022 was used in immunoprecipitation to show that CyP40 can not activate heat shock transcription factor 1"AuthorsZou J,Guo Y,Guettouche T,Smith DF,Voellmy R4.EndocrinologySusceptibility to diet-induced hepatic steatosis and glucocorticoid resistance in FK506-binding protein 52-deficient mice."PA3-022 was used in western blot to study the role of FK506-binding protein 52 in hepatic integrity and glucocorticoid responsiveness in mice"AuthorsWarrier M,Hinds TD,Ledford KJ,Cash HA,Patel PR,Bowman TA,Stechschulte LA,Yong W,Shou W,Najjar SM,Sanchez ER5.Molecular and cellular biologyThe hsp90-FKBP52 complex links the mineralocorticoid receptor to motor proteins and persists bound to the receptor in early nuclear events."PA3-022 was used in western blot to investigate the role of the heat shock protein 90-FKBP52 complex in the nucleus"AuthorsGaligniana MD,Erlejman AG,Monte M,Gomez-Sanchez C,Piwien-Pilipuk G

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