beta Actin Loading Control Monoclonal Antibody (BA3R), DyLight 488

货号：MA5-15739-D488

规格：50 µL

价格：2753

产品类型：内参抗体

品牌：Thermo Fisher

物种：人/小鼠/大鼠

宿主：小鼠

抗体亚型：其它

荧光染料：DyLight 488

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类型:	单抗	同型对照：	IgG2b
免疫原性：	Beta-actin N-terminal peptide.	用法：	1:20-1:100(ICC);1:20-1:100(IF)

Product Specific InformationMA5-15739-D488 detects beta actin from human, mouse, rat, rabbit, and chicken samples.MA5-15739-D488 has been successfully used in immunofluorescence. DyLight 488 has an excitation/emission of 493/518 nm.For visualization of actin filaments by immunofluorescence, fixation and permeabilization with methanol is required.Background/Target InformationBeta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

beta Actin Loading Control Antibody (MA5-15739-D488) in IFImmunofluorescent analysis of beta-actin (green) in HeLa cells. Cells were fixed with either 4% paraformaldehyde (PFA) for 15 minutes at room temperature followed by permeabilization with 0.5% Triton X-100 in PBS for 15 minutes at room temperature (left panel) or fixed and permeabilized with ice-cold methanol for 10 minutes at room temperature (right panel). Cells were blocked with 0.3% BSA in PBS for 15 minutes, and probed with a DyLight 488-conjugated beta-actin monoclonal antibody (Product # MA5-15739-D488) at a dilution of 1:20 overnight at 4C. Nuclei (blue) were stained with DAPI (Product # 62247). Images were taken on a ToxInsight Instrument at 20X magnification.

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参考文献：

1.PloS oneCaveolin-1 associated adenovirus entry into human corneal cells."MA5-15739-D488 was used in western blot to study HA-dvD endocytosis into corneal cells"AuthorsYousuf MA,Zhou X,Mukherjee S,Chintakuntlawar AV,Lee JY,Ramke M,Chodosh J,Rajaiya J2.Pathogens and diseaseElevated suppressor of cytokine signaling-1 (SOCS-1): a mechanism for dysregulated osteoclastogenesis in HIV transgenic rats."MA5-15739-D488 was used in western blot to investigate the mechanisms underlying dysregulated osteoclastogenesis in HIV"AuthorsLafferty MK,Fantry L,Bryant J,Jones O,Hammoud D,Weitzmann MN,Lewis GK,Garzino-Demo A,Reid W3.Developmental neuroscienceProgrammed cell death is impaired in the developing brain of FMR1 mutants."MA5-15739-D488 was used in western blot to assess apoptosis in the developing brain of FMR1 mutants"AuthorsCheng Y,Corbin JG,Levy RJ4.CarcinogenesisLoss of LKB1 and PTEN tumor suppressor genes in the ovarian surface epithelium induces papillary serous ovarian cancer."MA5-15739-D488 was used in western blot to study the role of LKB1 and PTEN loss in ovarian surface epithelial cells in the development of the papillary serous histotype of ovarian cancer"AuthorsTanwar PS,Mohapatra G,Chiang S,Engler DA,Zhang L,Kaneko-Tarui T,Ohguchi Y,Birrer MJ,Teixeira JM5.Journal of virologyPapillomavirus E6 PDZ interactions can be replaced by repression of p53 to promote episomal human papillomavirus genome maintenance."MA5-15739-D488 was used in western blot to study episomal HPV genome maintenance"AuthorsBrimer N,Vande Pol SB

技术参数

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