beta Actin Monoclonal Antibody (BA3R), DyLight 550

货号：MA5-15739-D550

规格：50 µL

价格：2753

产品类型：内参抗体

品牌：Thermo Fisher

物种：人/小鼠/大鼠

宿主：小鼠

抗体亚型：其它

荧光染料：DyLight 550

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类型:	单抗	同型对照：	IgG2b
免疫原性：	Beta-actin N-terminal peptide.	用法：	1:20-1:50(ICC);1:20-1:50(ICF)

Product Specific InformationMA5-15739-D550 has been successfully used for Immunofluorescence. DyLight 550 has an excitation/emission of 562/576nm.For visualization of actin filaments by immunofluorescence, fixation and permeabilization with methanol is required.Background/Target InformationBeta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

beta Actin Antibody (MA5-15739-D550) in IFImmunofluorescent analysis of beta-actin (red) in HeLa cells. Cells were fixed with either 4% paraformaldehyde (PFA) for 15 minutes at room temperature followed by permeabilization with 0.5% Triton X-100 in PBS for 15 minutes at room temperature (left panel) or fixed and permeabilized with ice-cold methanol for 10 minutes at room temperature (right panel). Cells were blocked with 0.3% BSA in PBS for 15 minutes, and probed with a DyLight 550-conjugated beta-actin monoclonal antibody (Product # MA5-15739-D550) at a dilution of 1:50 overnight at 4C. Nuclei (blue) were stained with DAPI (Product # 62247). Images were taken on a ToxInsight Instrument at 20X magnification.

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参考文献：

1.Cell death and diseaseEstradiol signaling mediates gender difference in visceral adiposity via autophagy.View published figure on By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2.Journal of virologyNF-κB and IRF1 Induce Endogenous Retrovirus K Expression via Interferon-Stimulated Response Elements in Its 5' Long Terminal Repeat.View published figure on By clicking the link above, you will be redirected to the Benchsci website.Disclaimer3.Nature biotechnologyA comparison of genetically matched cell lines reveals the equivalence of human iPSCs and ESCs.View published figure on By clicking the link above, you will be redirected to the Benchsci website.Disclaimer

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