beta Tubulin Monoclonal Antibody (2 28 33)

货号：32-2600

规格：100 µg

价格：4786

产品类型：流式抗体

品牌：Thermo Fisher

物种：人/小鼠/大鼠

宿主：小鼠

抗体亚型：其它

荧光染料：其它

类型:	单抗	同型对照：	IgG1, kappa
免疫原性：	Beta-tubulin from sea urchin (S. purpuratus) sperm.	用法：	1-3 µg/mL(ICC);1-3 µg/mL(IF);1 -3 µg/mL(WB)

Product Specific InformationThis antibody reacts with the ~50 kDa beta-tubulin and has been shown to bind to the two major and one of the minor beta-tubulin isotypes. Reactivity has been confirmed with mouse NIH3T3 fibroblast cells, rat brain, and mouse testis.Target InformationBeta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Beta-III tubulin is primarily expressed in neurons and may be involved in neurogenesis, axon guidance and maintenance. Mutations in the beta tubulin gene are the cause of congenital fibrosis of the extraocular muscles type 3. Beta-III tubulin was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues. Glutamate residues at the C-terminus of beta III tubulin can be glutamylated. The precise function of such modifications is unclear. Tubulin is phosphorylated on Ser-172 by CDK1 during cell cycle progression. Ser-172 phosphorylation inhibits tubulin incorporation into microtubules.Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55 kDa subunits: alpha and Beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton. Because beta-tubulin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

beta Tubulin Antibody (32-2600) in IFHuman iPSCs were cultured on glass slides under feeder-free conditions in StemPro® hESC Medium (Product # A1000701). Cells were fixed and permed with the Image-iT® Fixation/Permeabilization Kit (Product # R37602). Oct4 (green) expression was visualized using anti-Oct4 primary Ab and Alexa Fluor® 488 secondary Ab (Product # A-11034). Tubulin (red) expression was visualized using anti-tubulin primary Ab (Product # 32-2600) and Alexa Fluor® 594 secondary Ab (Product # A-11005). Nuclei (blue) were labeled with NucBlue™ Fixed Cell Stain (Product # R37606). Images were collected on the FLoid™ Cell Imaging Station (Product # 4471136). beta Tubulin Antibody (32-2600) in IFHuman iPSC Staining Human iPSCs were cultured on glass slides under feeder-free conditions in StemPro® hESC Medium (Product # A1000701). Cells were fixed and permed with the Image-iT® Fixation/Permeabilization Kit (Product # R37602). Oct4 (green) expression was visualized using anti-Oct4 primary Ab and Alexa Fluor® 488 secondary Ab (Product # A-11034). Tubulin (red) expression was visualized using anti-tubulin primary Ab (Product # 32-2600) and Alexa Fluor® 594 secondary Ab (Product # A-11005). Nuclei (blue) were labeled with NucBlue™ Fixed Cell Stain (Product # R37606). Images were collected on the FLoid™ Cell Imaging Station (Product # 4471136).

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参考文献：

1.The Journal of biological chemistryActivation of c-Abl Kinase Potentiates the Anti-myeloma Drug Lenalidomide by Promoting DDA1 Protein Recruitment to the CRL4 Ubiquitin Ligase."32-2600 was used in western blot to show that the Abl-DDB1-DDA1 axis reduces CRL4 substrate ubiquitination"AuthorsGao S,Geng C,Song T,Lin X,Liu J,Cai Z,Cang Y2.Reproductive biologyBrilliant cresyl blue staining does not present cytotoxic effects on human luteinized follicular cells, according to gene/protein expression, as well as to cytotoxicity tests."322600 was used in western blot to investigate the effects of Brilliant Cresyl Blue on human follicular cells exposed to Brilliant Cresyl Blue"AuthorsAlcoba DD,Schneider J,Arruda L,Martiny PB,Capp E,von Eye Corleta H,Brum IS3.Nature communicationsStarved epithelial cells uptake extracellular matrix for survival."32-2600 was used in Western Blotting to investigate the role of integrins and matrix adhesion in the survival of epithelial cells under growth restriction, where PI3K/mTOR signalling is decreased - a potential therapeutic target for cancers resistant to PI3K/mTOR inhibition."AuthorsMuranen T,Iwanicki MP,Curry NL,Hwang J,DuBois CD,Coloff JL,Hitchcock DS,Clish CB,Brugge JS,Kalaany NY4.Journal of neurotraumaStrong Correlation of Genome-Wide Expression after Traumatic Brain Injury In Vitro and In Vivo Implicates a Role for SORLA."32-2600 was used in western blot to study traumatic brain injury in vivo and in vitro to find a strong correlation of genome-wide expression of SORLA"AuthorsLamprecht MR,Elkin BS,Kesavabhotla K,Crary JF,Hammers JL,Huh JW,Raghupathi R,Morrison B5.OncotargetIdentification and validation of FGFR2 peptide for detection of early Barrett's neoplasia."32-2600 was used in Western Blotting to identify a novel peptide specific for FGFR2 that binds to the extra-cellular Ig-like domain of isoforms IIIb and IIIc in humans."AuthorsZhou J,He L,Pang Z,Appelman HD,Kuick R,Beer DG,Li M,Wang TD

技术参数

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