LysoTracker™ Red DND-99 - Special Packaging
货号： L7528
规格：20 x 50 µL
价格：5522
产品类型：细胞增殖活性检测
品牌：Thermo Fisher
物种：其它
宿主：其它
抗体亚型：其它
荧光染料：其它
| LysoTracker® Red DND-99 is a red-fluorescent dye for labeling and tracking acidic organelles in live cells.Features of all LysoTracker® probes include:• High selectivity for acidic organelles• Good retention after aldehyde fixation• Effective labeling of live cells at nanomolar concentrations• Live cell imaging• Excitation/Emission: 577/590 nmSimple, Highly Specific, One-Step Staining and Tracking of Acidic OrganellesThe LysoTracker® probes consist of a fluorophore linked to a weak base that is only partially protonated at neutral pH. This allows LysoTracker® probes to freely permeate cell membranes enabling them to label live cells. LysoTracker® probes are highly selective for acidic organelles, and unlike dinitrophenyl (DNP), LysoTracker® probes do not require use of a second antibody for detection.LysoTracker® probes are available in a variety of fluorescent colors. |
数据： |
| Live bovine pulmonary artery endothelial cells (BPAEC) labeled with LysoTracker® Red and Hoechst 33342.Live bovine pulmonary artery endothelial cells (BPAEC) were labeled with LysoTracker® Red DND-99 (Cat. no. L7528), a BODIPY® derivative, and Hoechst 33342 (Cat. no. H1399, H3570, H21492). The cells were then imaged by fluorescence and differential interference contrast (DIC) microscopy.Bovine pulmonary artery endothelial cells (BPAEC).Bovine pulmonary artery endothelial cells simultaneously stained with LysoTracker® Red DND-99 (Cat. No. L7528), a cell-permeant, fixable lysosomal stain, and with 5-(pentafluorobenzoylamino)fluorescein di-β-D-galactopyranoside (PFB-FDG, Cat. No. P11948), a fluorogenic substrate for beta-galactosidase. PFB-FDG is nonfluorescent until enzymatically hydrolyzed to green-fluorescent PFB-F. The center image demonstrates colocalization of the LysoTracker® Red DND-99 dye and PFB-F to the lysosomes. The left image was acquired with a bandpass filter set appropriate for fluorescein, the right image was acquired with a bandpass filter set appropriate for Texas Red® dye, and the center image was acquired with a double bandpass optical filter set appropriate for fluorescein and the Texas Red® dye. |
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