LC3B Antibody Kit for Autophagy
货号：L10382
规格：1 kit
价格：5340
产品类型：流式抗体
品牌：Thermo Fisher
物种：其它
宿主：其它
抗体亚型：其它
荧光染料：其它
| LC3 自噬抗体试剂盒包含兔抗 LC3B 多克隆抗体，已经过验证可用于荧光显微镜检查和高内涵成像及分析。还包含二磷酸氯喹——使用这种化合物处理后，正常自噬通量被破坏，进而导致自噬体积累。 |
数据： |
| Autophagy detected in Human skeletal muscle cellsGibco® Human skeletal muscle cells were treated with 30uM Chloroquine overnight. The following day the cells were fixed and permeabilized and labeled with 0.5ug/mL anti-LC3B with a goat anti mouse Alexa Fluor® 555 secondary (Red) Cells were counter stained with 1ug/mL Hoechst 33342 (Blue) Images were acquired with a BD Pathway 855 High content imagerUsing anti-LC3B and the Click-iT® technology autophagy detected and glycoproteins labeled in HeLa cellsHeLa cells were fed 50uM ManNAz under regular growth conditions, after 24 hours the media was changed and 30uM chloroquine was added. The following day cells were fixed and permeabilized. ManNaz was detected using Click-iT® reactions with an alkyne Alexa Fluor® 488 (Green). Cells were labeled with 0.5ug/mL anti-LC3B with a goat anti rabbit Alexa Fluor® 647 secondary (red) and 1ug/mL Hoechst 33342 (Blue). Cells were imaged on a Zeiss LSM 710 confocal microscope. |
相关产品： |
| ▪Premo™ Autophagy Sensor RFP-p62 Kit（货号#P36241）▪ Premo™ Autophagy Sensor GFP-p62 Kit（货号#P36240）▪Premo™ Autophagy Sensor GFP-LC3B Kit（货号#P36235）▪Premo™ Autophagy Sensor RFP-LC3B Kit（货号#P36236）▪Premo™ Autophagy Tandem Sensor RFP-GFP-LC3B Kit（货号#P36239） |
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