End-It™ DNA End-Repair Kit/End-It™DNA末端修复试剂盒（停产））
货号：ER81050,ER0720
规格：50 rxns,20 rxns
价格：3126,1449
产品类型：二代测序-建库
品牌：Lucigen
The End-It™ DNA End-Repair Kit is used to convert DNA with damaged or incompatible 5′-protruding and/or 3′-protruding ends to 5′-phosphorylated, blunt-end DNA for fast and efficient blunt-end ligation into plasmid, cosmid, fosmid, BAC, other cloning vectors, or nextgen DNA sequencing adaptors. End-repaired DNA can be efficiently and rapidly blunt-end ligated into DNA cloning vectors or to next-gen DNA sequencing adaptors using Lucigen’s Fast-Link™ DNA Ligation Kits. End-It DNA末端-修复试剂盒用于将带有受损或不相容的5，-突出和/或3，-突出末端的DNA转化为5，-磷酸化的平末端DNA，用于随后添加下一代DNA测序接头，或用于克隆。用End-It试剂盒处理DNA后，可以使用Fast-Link DNA连接试剂盒（Lucigen）进行快速有效的平末端连接. |
| 优点： |
| ▪ Complete conversion of DNA into blunt-ended, phosphorylated DNA▪ Repair sheared, nebulized or restriction enzyme-digested genomic DNA ▪ Prepare templates for NGS (next-generation sequencing) or shotgun library preparation▪ 将DNA完全转化为末端钝化的磷酸化DNA▪ 修复剪切，雾化或限制性内切酶消化的基因组DNA▪ 准备用于NGS（二代测序）或shot弹枪文库准备的模板 |
| 组成成分： |
| ▪ End-Repair Enzyme Mix：-20°C▪ End-Repair 10X Buffer：-20°C▪ ATP：-20°C ▪ dNTPs：-20°C |
技术参数 产品优点 - Complete conversion of DNA into blunt-ended, phosphorylated DNA
- Repair sheared, nebulized or restriction enzyme-digested genomic DNA
- Prepare templates for NGS (next-generation sequencing) or shotgun library preparation
- 将DNA完全转化为末端钝化的磷酸化DNA
- 修复剪切，雾化或限制性内切酶消化的基因组DNA
- 准备用于NGS（二代测序）或shot弹枪文库准备的模板
产品应用 - Prepare sheared, nebulized, or restriction enzyme digested genomic DNA for ligation of next-gen DNA sequencing adaptors.
- Prepare double-stranded cDNA, produced from cellular RNA transcripts, for ligation of next-gen DNA sequencing adaptors.
- Prepare sheared, nebulized, or restriction enzyme digested DNA for blunt-end ligation into plasmid, cosmid, fosmid, or BAC vectors.
- Prepare DNA amplified by PCR, containing A-overhangs, for efficient and cost-effective blunt-end cloning.
- 在连接下一代测序接头之前，对收到酶或机械剪切受损的DNA或cDNA进行修复
- 在克隆λ质粒、粘粒、fosmid或BAC载体之前，对收到酶或机械剪切损伤的DNA、cDNA或带有A突出端的PCR扩增子进行修复
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