Exonuclease VII, (Exo VII) derived from E. coli, has a high enzymatic specificity for singlestranded DNA (ssDNA) and exhibits both 5′→3′and 3′→5′ exonuclease activities. It is especially useful for rapid removal of single stranded oligonucleotide primers from a completed PCR reaction when different primers are required for subsequent PCR reactions. Exo VII digestion of ssDNA works in Mg2+-free buffer containing EDTA. Exonuclease VII对ssDNA具有高特异性酶活性，并且表现出5’→3’和3’→5’两个方向的核酸外切酶活性。当PCR后续不需要不同的引物时，它可用于从已完成的扩增反应中快速去除单链寡核苷酸引物。核酸外切酶VII消化ssDNA不需要镁离子的存在，可在不含镁离子的EDTA溶液中起作用。
优点：
▪ Remove single-stranded oligonucleotide primers after PCR▪ Minimize the effect of primers left over from previous PCR amplifications
组成成分：
▪ Exonuclease VII (10 U/μL)：-20°C▪ 5X Exonuclease VII Buffer：-20°C
参考文献：
1. Li, H. et al., (1991) Nucl. Acids Res. 19, 3139.
技术参数
产品优点 - Remove single-stranded oligonucleotide primers after PCR - Minimize the effect of primers left over from previous PCR amplifications - PCR反应后去除单链寡核苷酸引物 - 最大限度地减少先前PCR反应残留引物的影响
产品应用 - Removal of primers from completed PCR reactions. - Minimize the effect of primers left over from previous PCR reactions.
QQ:1749072012