Exonuclease VII / 外切酶VII-Bio-Rad-Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱

Exonuclease VII / 外切酶VII

2024-10-17

Exonuclease VII / 外切酶VII

货号：EN510250

规格：250 U 10 U/µL

价格：3776

产品类型：核酸酶和抑制剂

品牌：Lucigen

Exonuclease VII, (Exo VII) derived from E. coli, has a high enzymatic specificity for singlestranded DNA (ssDNA) and exhibits both 5′→3′and 3′→5′ exonuclease activities. It is especially useful for rapid removal of single stranded oligonucleotide primers from a completed PCR reaction when different primers are required for subsequent PCR reactions. Exo VII digestion of ssDNA works in Mg²⁺-free buffer containing EDTA. Exonuclease VII对ssDNA具有高特异性酶活性，并且表现出5’→3’和3’→5’两个方向的核酸外切酶活性。当PCR后续不需要不同的引物时，它可用于从已完成的扩增反应中快速去除单链寡核苷酸引物。核酸外切酶VII消化ssDNA不需要镁离子的存在，可在不含镁离子的EDTA溶液中起作用。

优点：

▪ Remove single-stranded oligonucleotide primers after PCR▪ Minimize the effect of primers left over from previous PCR amplifications

组成成分：

▪ Exonuclease VII (10 U/μL)：-20°C▪ 5X Exonuclease VII Buffer：-20°C

参考文献：

1. Li, H. et al., (1991) Nucl. Acids Res. 19, 3139.

技术参数

产品优点 - Remove single-stranded oligonucleotide primers after PCR - Minimize the effect of primers left over from previous PCR amplifications - PCR反应后去除单链寡核苷酸引物 - 最大限度地减少先前PCR反应残留引物的影响

产品应用 - Removal of primers from completed PCR reactions. - Minimize the effect of primers left over from previous PCR reactions.

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Exonuclease VII / 外切酶VII

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