PDGFRA Polyclonal Antibody
货号：PA5-16742
规格：500 µL
价格：0
产品类型：免疫组化一抗
品牌：eBioscience
物种：人/小鼠
宿主：兔
抗体亚型：IgG
荧光染料：Purified
类型: | 多抗 | 同型对照： | |
浓度： | | 用法： | 1：100（IHC (P)） |
免疫原性： | A synthetic peptide derived from C-terminal of human PDGFR alpha |
产品详细信息
PA5-16742 targets PDGFR alpha in IHC (P) applications and shows reactivity with Human and mouse samples.The PA5-16742 immunogen is a synthetic peptide derived from C-terminal of human PDGFR alpha.
靶标信息
PDGFR (Platelet Derived Growth Factor Receptor) exhibits two different isoforms, alpha; and beta; coded by two different genes. PDGF A receptors are expressed in oligodendrocyte progenitor cells and PDGF B receptors are expressed on neurons. This antibody is specific for PDGFR, alpha; and does not react with PDGFR, beta.
数据 |
| PDGFRA Antibody (PA5-16742) in IHC (P) Immunohistochemistry analysis of PDGFR alpha showing staining in the membrane and cytoplasm of paraffin-embedded mouse kidney tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a PDGFR alpha Rabbit Polyclonal Antibody (Product # PA5-16742) diluted in 3% BSA-PBS at a dilution of 1:100 for 1 hour at 37ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting. PDGFRA Antibody (PA5-16742) in IHC Formalin-fixed, paraffin-embedded human angiosarcoma stained with PDGFR alpha antibody using peroxidase-conjugate and AEC. Note cytoplasmic staining of tumor cells. |
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| 二抗荧光二抗免疫组化一抗标签抗体 |
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