Pierce Streptavidin Coated High Sensitivity Plates, Clear, 8-Well Strip 链霉亲和素包被高灵敏度8孔透明板

2024-10-17

Pierce Streptavidin Coated High Sensitivity Plates, Clear, 8-Well Strip 链霉亲和素包被高灵敏度8孔透明板

货号:15520

规格:5 plates

价格:5128

产品类型:ELISA检测

品牌:Thermo Fisher

物种:其它

宿主:其它

抗体亚型:其它

荧光染料:其它

Thermo Scientific Pierce 链霉亲和素包被高灵敏度板(透明,8 孔联排)是生物素结合微孔板,进行了包被,以在针对低水平靶标分析物的测定中尽可能提高生物素化探针捕获效率并控制背景。Thermo Scientific Pierce 包被板使用具有专利的表面活化技术进行了包被,以尽可能提高活性链霉亲和素生物素结合蛋白的包被效率。该高灵敏度板可以检测低浓度 (ng/mL) 生物素化抗体和其他蛋白。无论是通过荧光、比色还是化学发光方法检测信号,在针对具有低水平靶标的任何样品开发 ELISA 时这些板均能使其变得容易。链霉亲和素是最常用且广泛可用的生物素结合蛋白,可用于研究方法。链霉亲和素的等电点为 5 至 6,因此非特异性相互作用较低。
特点:
-灵敏—可检测低至 5 ng/mL 生物素化 IgG-特异性—具有高信噪比的低背景-通用—可使用荧光、化学发光或比色检测-稳健—宽动态范围-即用型—板在提供时进行了预封闭,以节省时间
数据:

Detect low concentrations of biotinlyated target molecules using High Sensitivity Coated PlatesBiotinylated rabbit IgG was incubated for 30 minutes in streptavidin-coated high sensitivity plates (HS) or streptavidin-coated standard binding capacity (SBC) microplates. Plates were washed three times with Tris-buffered saline and incubated with goat anti-rabbit IgG conjugated to horseradish peroxidase (100ng/mL) for 30 minutes. Plates were washed and incubated with Thermo Scientific QuantaBlu Fluorogenic Peroxidase Substrate (Part No. 15169). The fluorescent signal was measured at 430nm.

Comparison of Streptavidin Coated Plate typesDetection Ranges were determined using Thermo Scientific QuantaBlu Fluorogenic Peroxidase Substrate.

技术参数
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