5' TAMRA Amidite (N-TAMRA-Piperidinyl), 6-Carboxy Single Isomer
货号：BNS-5027B-100,BNS-5027B-250,BNS-5027B-50
规格：100 µmol,250 mg,50 µmol
价格：3165.75,11256,1758.75
产品类型：核酸合成
品牌：LGC Biosearch Technologies
| Tetramethylrhodamine CE phosphoramidite (TAMRA Amidite) is used for the labeling of oligonucleotides and fluorogenic probes. This product is prepared using the pure 6-carboxytetramethylrhodamine isomer and can be added only at the 5' terminus of the oligo, thereby terminating the synthesis. For best result we recommend a 10 minute coupling routine and an additional capping step after oxidation. Rhodamine dyes are susceptible to degradation by strong bases such as concentrated NH4OH, we suggest using t-butylamine/H2O for deprotection and cleavage. |
| 特性： |
| - Chemical Name:1-[(Tetramethylrhodamin-6-yl)carbonyl]piperidin-4-yl-2-cyanoethyl-N,N-diisopropyl phosphoramidite- Formula:C39H48N5O6P- Molecular Weight:713.33- Appearance:pink solid- Absorption Maximum (Lambda Max):555- Extinction Coefficient at Lambda max:90000- Extinction Coefficient at 260 nm:31980- Fluorescence Maximum:576 |
| 用途： |
| Synthesis conditions:Prior to dilution ensure that all product is at the bottom of the vial. Dilute to the recommended concentration and mix thoroughly in the sealed vial to ensure that all contents are dissolved. Product should only be used for DNA synthesis with standard reagents ( i.e. Ethylthio Tetrazole, 0.02M Iodoine/THF).Dilution:100 µmol/mLCoupling:10-minute couplingDeprotection conditions:When using fast deprotecting amidites (eg. C-Ac, G-DMF, G-PAC) use t-butylamine/Water (1:3) for 6 hours at 65°C. When using standard amidites (eg. C-Bz, G-iBu) use t-butylamine/Water (1:3) overnight (12-15 hours) at 65°C. Strong bases, such as concentrated ammonia cause degradation of rhodamine dyes.Image of cleaved and deprotected structure: The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:575.56 |
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