CD8a Monoclonal Antibody (RPA-T8), PE-eFluor 610, eBioscience™

货号：61-0088-41,61-0088-42

规格：25tests,100tests

价格：1244,3107

产品类型：流式抗体

品牌：Thermo Fisher

抗原：CD8a

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：RPA-T8

荧光染料：PE-eFluor 610

抗体类型:	流式抗体	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-eFluor 610, eBioscience
浓度：	5 µL/Test	用法：	5 µL (0.06 µg)/test (Flow）

产品详细信息Description:The RPA-T8 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T-cell development and activation of mature T cells.Applications Reported: This RPA-T8 antibody has been reported for use in flow cytometric analysis.Applications Tested: This RPA-T8 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.Target InformationCD8 molecule is composed of two chains termed alpha and beta. CD8 is found on a T cell subset of normal cytotoxic / suppressor cells which make up approximately 20 to 35% of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer cells, 80% of thymocytes, on a subpopulation of 30% of peripheral blood null cells and 15 to 30% of bone marrow cells.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD8a Antibody (61-0088-41) in FlowStaining of normal human peripheral blood cells with Anti-Human CD3 APC (Product # 17-0037-42) and Mouse IgG1 K Isotype Control PE-eFluor® 610 (Product # 61-4714-82) (left) or Anti-Human CD8a PE-eFluor® 610 (right). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.Development (Cambridge, England)Neural-specific deletion of the focal adhesion adaptor protein paxillin slows migration speed and delays cortical layer formation."61-0088 was used in Western Blotting to study the roles of paxillin and Hic-5 in cortical development."AuthorsRashid M,Belmont J,Carpenter D,Turner CE,Olson EC2.Stem cells internationalUmbilical Cord-Derived Mesenchymal Stem Cells Suppress Autophagy of T Cells in Patients with Systemic Lupus Erythematosus via Transfer of Mitochondria."Published figure using CD8a monoclonal antibody (Product # 61-0088-42) in Flow Cytometry"3.Stem cells and developmentImmunological Properties of Neural Crest Cells Derived from Human Induced Pluripotent Stem Cells."Published figure using CD8a monoclonal antibody (Product # 61-0088-42) in Flow Cytometry"

技术参数

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