3'-DMT-dG(iBu) Synthesis Column; 1000 Å
货号：CG1-1200i-1,CG1-1200i-2,CG1-1200i-5
规格：1 µmol,200 nmol,50 nmol
价格：84.42,70.35,56.28
产品类型：核酸合成
品牌：LGC Biosearch Technologies
| This column is packed with 3'-DMT-dG(iBu)-Suc-CPG, an inverse linkage CPG used to create a 3'-3' linkage on the end of an oligonucleotide. An inverted 3'-3' linkage is desirable when needing to block polymerase extension through the 3'-terminus of a PCR probe, additionally oligonucleotides with inverted 3'-linkages are used for some sequencing techniques. The 1000 Å pore size is best suited for the synthesis of DNA sequences up to 100-mers in length. |
| 特性： |
| Chemical Name: 3'-DMT-dG(iBu)-Suc-CPG; 500 ÅAppearance: White PowderExtinction Coefficient at 260 nm: 12010 |
| 产品用途： |
| Protecting group deblock conditions: 3'DMT groups detritylate slowly. Increase detritylation time by 50%.Cleavage conditions: Use concentrated ammonia for 90 minutes at 25 °C when using standard amidites or 1:1 ammonia:methylamine (AMA) for 25 minutes at 25 °C.Deprotection conditions: Use concentrated ammonia for 5 hours at 60 °C.Image of cleaved and deprotected structure:The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is: 329.2 |
| 数据： |
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QQ:1749072012