DABCYL-C3 Synthesis Column (O-DMT-N-DABCYL-3-aminopropan-1,2-diol-Suc-CPG); 500 Å
货号:CG5-5026-1,CG5-5026-2,CG5-5026-5
规格:1 µmol,200 nmol,50 nmol
价格:562.8,281.4,211.05
产品类型:核酸合成
品牌:LGC Biosearch Technologies
| This synthesis column, packed with 5’-DMT mdC-DABCYL 3’-Suc support, allows for 3' labeling of molecular beacons and acts as a quencher moiety. Our DABCYL C3 CPG is synthesized with a three carbon linker arm and is attached to the solid support via a succinate linkage. DABCYL is used as a quencher for fluorophore groups such as fluorescein, TAMRA and JOE. DABCYL absorbs between 400 to 525 nm with maximum quenching at 474 nm. |
| 特性: |
| ▪ Chemical Name:DABCYL-C3-CPG (O-DMT-N-DABCYL-3-aminopropan-1,2-diol-Suc-CPG); 500 Å▪ Formula:C18H20N4O3 ▪ Molecular Weight:340.14▪ Appearance:orange powder▪ Absorption Maximum (Lambda Max):474 |
| 产品用途: |
| Protecting group deblock conditions:Standard detritylationCleavage conditions:This support may be cleaved using concentrated NH4OH or t-butylamine/H2O (TAMRA and other rhodamine-based dyes require deprotection with t-butylamine).Deprotection conditions:If you are using fast deprotecting amidites [i.e., C(Ac), G(DMF), G(PAC)], deprotect in concentrated NH4OH for 1 hour at 60 °C.
-If you are using standard amidites [i.e., A(Bz), G(iBu)], deprotect in concentrated NH4OH for 5 hours at 60 °C.
-If you are using base sensitive fluorophores (i.e., TAMRA or other rhodamine-based dyes), we recommend deprotecting oligonucleotides in t-butylamine/H2O for 12 hours at 60 °C.The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:404.36 |
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