IL-12/IL-23 p40 Monoclonal Antibody (C17.8)

2024-10-18

IL-12/IL-23 p40 Monoclonal Antibody (C17.8)

货号:13-7123-81,13-7123-85

规格:50μg,500μg

价格:1399,3964

产品类型:抗体和染料

品牌:eBioscience

物种:小鼠

宿主:大鼠

抗体亚型:IgG

克隆号:C17.8

荧光染料:Biotin

类型:单抗同型对照:Rat IgG2a kappa Isotype Control (eBR2a), Biotin
浓度:0.5 mg/mL用法:0.5-2 µg/mL (ELISA)
产品详细信息Description: The C17.8 antibody reacts with the p40 subunit of IL-12 and IL-23. The C17.8 antibody has been found to neutralize IL-12 and IL-23 bioactivity. For specific neutralization of IL-23 only, please see clone G23-8 (anti-mouse IL-23 p19). IL-12 is a 70 kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and is secreted by activated monocytes, macrophages, and dendritic cells. IL-23 is a heterodimer composed of the disulfide-linked p19 protein and p40 protein, expressed by activated dendritic cells.Applications Reported: The C17.8 antibody has been reported for use in IP, Western, ELISA, neutralization, and intracellular staining for flow cytometric analysis.Applications Tested: The biotinylated C17.8 antibody has been tested as the detection antibody in three different sandwich ELISAs, for measurement of mouse IL-12 p70, mouse IL-23, or mouse IL-12 p40 total.For mouse IL-12 p70 ELISA: The biotinylated C17.8 antibody has been tested as the detection antibody in sandwich ELISA for analysis of mouse IL-12 p70, in combination with the purified C18.2 antibody (14-7122) for capture and recombinant mouse IL-12 p70 (39-8121) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2,000 pg/mL - 15 pg/mL should be included in each ELISA plate. Super AquaBlue or ABTS are suitable substrates.For mouse IL-23 ELISA: The biotinylated C17.8 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse IL-23 protein levels, in combination with the purified G23-8 antibody (14-7232) for capture and recombinant mouse IL-23 (39-8231) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.25 - 1 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 4,000 pg/mL - 30 pg/mL should be included in each ELISA plate. Note: TMB (00-4201), rather than ABTS, should be used as the substrate.For mouse IL-12 p40 Total ELISA: The biotinylated C17.8 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse total p40 (e.g., p40 monomer, p40 dimer, p40/p35, and p40/p19), in combination with the purified C15.6 antibody (14-7125) for capture and recombinant mouse IL-12 p40 (39-8122), IL-12 p70 (39-8121) or mouse IL-23 (39-8231) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2,000 pg/mL - 15 pg/mL should be included in each ELISA plate. Super AquaBlue or ABTS are suitable substrates.Filtration: 0.2 µm post-manufacturing filtered.靶标信息IL-12 is an interleukin produced by dendritic cells, neutrophils, macrophages and human lymphoblastoid cells as a result of antigenic stimulation. IL-12 is a heterodimeric cytokine encoded by IL-12A (p35) and IL-12B (p40). IL-23 is a heterodimeric cytokine that shares a subunit with IL-12 (IL-12B) along with an IL-23A (IL-23p19) subunit. Functionally, IL-12 increases the expression of vascular endothelial adhesion molecule-1 on the endothelium, directs leukocytes towards tumors and facilitates the ischemic-hemorrhagic necrosis of the tumor tissue. In comparison, IL-23 plays an important role in stimulating memory T-cells, and is required for the induction, expansion, maintenance and downstream effector functions of Th17 cells, which play a vital role in upregulating neutrophil chemokines and various pro-inflammatory cytokines.
数据
IL-12/IL-23 p40 Antibody (13-7123-81)Nature communications 2018 -Published figure using IL-12/IL-23 p40 monoclonal antibody (Product # 13-7123-81)
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参考文献:

1.TheranosticsAbsence of GdX/UBL4A Protects against Inflammatory Diseases by Regulating NF-кB Signaling in Macrophages and Dendritic Cells."13-7123 was used in an ELISA assay to investigate whether ubiquitin-like protein 4A has a role during the innate immune response."AuthorsLiu C,Zhou Y,Li M,Wang Y,Yang L,Yang S,Feng Y,Wang Y,Wang Y,Ren F,Li J,Dong Z,Chin YE,Fu X,Wu L,Chang Z2.Scientific reportsAnalysis of the Trichuris suis excretory/secretory proteins as a function of life cycle stage and their immunomodulatory properties."13-7123 was used in an ELISA assay to analyse the Trichuris suis excretory/secretory proteins as a function of life cycle stage and their immunomodulatory properties."AuthorsLeroux LP,Nasr M,Valanparambil R,Tam M,Rosa BA,Siciliani E,Hill DE,Zarlenga DS,Jaramillo M,Weinstock JV,Geary TG,Stevenson MM,Urban JF,Mitreva M,Jardim A3.Cellular and molecular immunologyp38α has an important role in antigen cross-presentation by dendritic cells."13-7123 was used in an ELISA assay to study whether the p38 signalling pathway affects the important antigen presentation function of dendritic cells."AuthorsZhou Y,Wu J,Liu C,Guo X,Zhu X,Yao Y,Jiao Y,He P,Han J,Wu L4.Mucosal immunologyIntestinal bacteria induce TSLP to promote mutualistic T-cell responses."13-7123 was used in an ELISA assay to show that steady-state thymic stromal lymphopoietin expression requires intestinal bacteria and has an important role in limiting the expansion T helper cells in the colon."AuthorsMosconi I,Geuking MB,Zaiss MM,Massacand JC,Aschwanden C,Kwong Chung CK,McCoy KD,Harris NL5.European journal of immunologyNrf2 is essential for cholesterol crystal-induced inflammasome activation and exacerbation of atherosclerosis."13-7123 was used in an ELISA assay to identify NF-E2-related 2 as an essential positive regulator of inflammasome activation and IL-1-mediated vascular inflammation."AuthorsFreigang S,Ampenberger F,Spohn G,Heer S,Shamshiev AT,Kisielow J,Hersberger M,Yamamoto M,Bachmann MF,Kopf M
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