IL-12/IL-23 p40 Human ELISA Kit/人IL-12/IL-23 p40 ELISA试剂盒-Bio-Rad-Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱

IL-12/IL-23 p40 Human ELISA Kit/人IL-12/IL-23 p40 ELISA试剂盒

2024-10-18

IL-12/IL-23 p40 Human ELISA Kit/人IL-12/IL-23 p40 ELISA试剂盒

货号：BMS2013,BMS2013TEN

规格：96 Tests,10X96 Tests

价格：6120,35475

产品类型：Platinum ELISA

品牌：Thermo Fisher

物种：人

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	<10 pg/mL
检测范围	31.3-2,000 pg/mL
样本类型/体积	血清 50 μL 血浆 50 μL 上清液 50 μL
操作时间	1 hr 20 min
测试时间	4 hr 10 min
Homogenous (no wash)	No
批间变异系数（CV）	6.4%
批内变异系数（CV）	4.7%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	10 x 96 tests
组份	- 预包被96孔板 - 标准品 - 样品稀释液 - 测定稀释浓缩液 - 生物素化检测抗体 - SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖
储存温度	2-8℃
Protein name	IL-12/IL-23 p40
Protein aliases	CLMF p40, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL-12 subunit p40, IL-12B, IL-23 subunit alpha, IL-23-A, IL-23p19, IL12, subunit p40, interleukin 12, p40, interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40), interleukin 23 p19 subunit, interleukin 23, alpha subunit p19, interleukin-12 beta chain, Interleukin-12 subunit beta, Interleukin-23 subunit alpha, Interleukin-23 subunit p19, interleukin-six, G-CSF related factor, JKA3 induced upon T-cell activation, natural killer cell stimulatory factor, 40 kD subunit, NK cell stimulatory factor chain 2, NKSF2
物种（Tested）	人
试剂盒类型	Sandwich ELISA Kit
标记（染料）	HRP
检测抗体偶联物	Biotin
Gene aliases	CLMF, CLMF2, IL-12B, IL-23, IL-23A, IL12B, IL23A, IL23P19, IMD28, IMD29, NKSF, NKSF2, P19, SGRF, UNQ2498/PRO5798
Gene ID	(Human) 3593,(Human) 51561
Gene symbol	IL12B, IL23A
UniProt ID	(Human) P29460,(Human) Q9NPF7

The Human Interleukin-12/Interleukin-23 p40 subunit (Hu IL-12/IL-23 p40) ELISA quantitates Hu IL-12/IL-23 p40 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-12/IL-23 p40.人白介素12 /白介素23 p40亚基（人IL-12 / IL-23 p40）ELISA定量了人血清，血浆，缓冲液或细胞培养基中的人IL-12 / IL-23 p40。该检测试剂盒将专门识别天然和重组的人IL-12 / IL-23 p40。Principle of the method 方法原理The Human IL-12/IL-23 p40 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人IL-12 / IL-23 p40固相夹心ELISA（酶联免疫吸附测定）用于测量匹配的抗体对之间结合的靶标量。已在提供的微孔板的孔中预包被了靶标特异性抗体。然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。有关验证的更多信息，请参见手册。Target information 靶标信息IL-12 is an interleukin produced by dendritic cells, neutrophils, macrophages and human lymphoblastoid cells as a result of antigenic stimulation. IL-12 is a heterodimeric cytokine encoded by IL-12A (p35) and IL-12B (p40). IL-23 is a heterodimeric cytokine that shares a subunit with IL-12 (IL-12B) along with an IL-23A (IL-23p19) subunit. Functionally, IL-12 increases the expression of vascular endothelial adhesion molecule-1 on the endothelium, directs leukocytes towards tumors and facilitates the ischemic-hemorrhagic necrosis of the tumor tissue. In comparison, IL-23 plays an important role in stimulating memory T-cells, and is required for the induction, expansion, maintenance and downstream effector functions of Th17 cells, which play a vital role in upregulating neutrophil chemokines and various pro-inflammatory cytokines.IL-12是由于抗原刺激而由树突状细胞，嗜中性粒细胞，巨噬细胞和人淋巴母细胞产生的白介素。 IL-12是由IL-12A（p35）和IL-12B（p40）编码的异二聚体细胞因子。 IL-23是一种异二聚体细胞因子，与IL-12（IL-12B）亚基以及IL-12A（IL-12p19）亚基共享一个亚基。在功能上，IL-12增加了血管内皮粘附分子-1在内皮上的表达，将白细胞引向肿瘤，并促进了肿瘤组织的缺血性出血性坏死。相比之下，IL-23在刺激记忆性T细胞中起重要作用，并且是Th17细胞的诱导，扩增，维持和下游效应子功能所必需的，Th17细胞在上调中性粒细胞趋化因子和各种促炎性细胞因子中起着至关重要的作用。。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

数据：

IL-12/IL-23 p40 Human ELISA KitRepresentative standard curve for Human IL-12/IL-23 p40 ELISA

技术参数

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