Mouse IgG2a kappa Isotype Control (eBM2a), eFluor 660
货号：50-4724-80
规格：25 μg
价格：1156
产品类型：小鼠同型对照
品牌：Thermo Fisher
物种：其它
宿主：小鼠
抗体亚型：IgG2a, kappa
克隆号：eBM2a
荧光染料：eFluor 660
| 类型: | | 同型对照： | |
| 浓度： | 0.2 mg/mL | 用法： | Assay-Dependent (Ctrl)；Assay-Dependent (Flow) |
产品详细信息Description: This is a monoclonal mouse IgG2a antibody. It is used as an isotype control for mouse IgG2a antibodies.Applications Reported: This eBM2a antibody has been reported for use in immunocytochemistry, immunohistochemistry, and flow cytometric analysis.Applications Tested: This eBM2a antibody has been tested by flow cytometric analysis of normal human peripheral blood cells or mouse splenocytes. Use at the same concentration as the experimental antibody.eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
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