产品优点 • 100% activity of all FastDigest enzymes in the universal buffer • 100% buffer compatibility with downstream applications • Complete digestion in 5–15 minutes • Direct loading on gels • No star activity • 176 FastDigest enzymes available •通用缓冲液中所有FastDigest酶100％活性 •与下游应用程序具有100％的缓冲液兼容性 •5-15分钟内完全消化 •可直接加样到凝胶上 •没有星号活性 •176种FastDigest酶可用

产品应用 • Molecular cloning • Restriction site mapping • Genotyping • Southern Blot • Restriction fragment length polymorphism (RFLP) • SNP analysis Note: FastDigest BplI reguires only Mg2+ for its activity, but is stimulated by SAM. 0.05 mM S-adenosylmethionine gives more than a 100-fold increase in FastDigest BplI activity. Still, complete cleavage of some substrates by FastDigest BplI is difficult to achieve. The FastDigest Green Buffer offers the same high performance in DNA digestion and downstream applications as the colorless FastDigest Buffer. For applications that require product analysis by fluorescence excitation (e.g. concentration measurements in UV light) the colorless FastDigest Buffer is recommended. For methylation sensitivity, refer to product specifications. Note: FastDigest BplI reguires only Mg2+ for its activity, but is stimulated by SAM. 0.05 mM S-adenosylmethionine gives more than a 100-fold increase in FastDigest BplI activity. Still, complete cleavage of some substrates by FastDigest BplI is difficult to achieve. The FastDigest Green Buffer offers the same high performance in DNA digestion and downstream applications as the colorless FastDigest Buffer. For applications that require product analysis by fluorescence excitation (e.g. concentration measurements in UV light) the colorless FastDigest Buffer is recommended. For methylation sensitivity, refer to product specifications. •分子克隆 •限制性位点比对 •基因分型 •Southern印迹 •限制性片段长度多态性（RFLP） •SNP（单核苷酸多态性） 注意：FastDigest BplI仅要求Mg2+具有活性，但受SAM刺激。 0.05 mM S-腺苷甲硫氨酸可使FastDigest BplI活性提高100倍以上。 但是，很难通过FastDigest BplI完全切割某些底物。 FastDigest绿色缓冲液在DNA消化和下游应用中提供与无色FastDigest缓冲液相同的高性能。 对于需要通过荧光激发进行产品分析的应用（例如，紫外光中的浓度测量），建议使用无色的FastDigest缓冲液。 有关甲基化敏感性信息，请参阅产品说明。