TransformAid Bacterial Transformation Kit

货号：K2711

规格：40 transformations

价格：847

产品类型：分子生物学

品牌：Thermo Fisher

描述Thermo Scientific TransformAid Bacterial Transformation Kit uses a novel method for rapid preparation of chemically-competentE. colicells from overnight bacterial cultures or bacterial colonies. The key component of this system is the unique T-solution, which produces competent cells in a few easy steps. The quick and convenient procedure guarantees transformation efficiencies of more than 107transformants per µg of plasmid DNA, ideal for routine cloning experiments. The option to use bacterial colonies for preparation of competent cells adds flexibility to any cloning experiment.The TransformAid Bacterial Transformation Kit can be used with mostE. colistrains commonly used for cloning: JM107, JM109, XL1-Blue, SURE, TOPP2, W3110, NM527, AD494, CJ236, GM2163, C600, BL21, M15, NM522, HB101, ER2267.Highlights• Efficient—more than 107transformants per µg of plasmid DNA• Practical—E. colicompetent cells can be prepared from an overnight culture or from bacterial colonies• Fast—less than 1 hour from the overnight bacterial culture to cell plating. Starting from bacterial colonies, the procedure requires only 2.5 hours• Easy—all procedures are performed on ice on your bench. Brief centrifugations are carried out in regular microcentrifugesApplications• Routine cloning experiments• Blunt-end cloning• TA cloningIncludes• C-Medium• T-Solution (A)• T-Solution (B)• Detailed ProtocolNoteCompetent cells prepared with TransformAid Bacterial Transformation Kit are suitable for direct-use only. Freezing down and storage at -70°C is not recommended.Transformation with the TransformAid Bacterial Transformation Kit

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