IPTG, dioxane-free
货号：R0392,R0393
规格：5 g,5 x 5 g
价格：1356,4963
产品类型：分子生物学
品牌：Thermo Fisher
描述Thermo Scientific IPTG (isopropyl-beta-D-thiogalactopyranoside) is a highly stable synthetic analog of lactose. It inactivates thelacrepressor and induces synthesis of beta-galactosidase, an enzyme that promotes lactose utilization. IPTG is used to induce expression of cloned genes under control of thelacoperon (seeReference 1). It is used in conjunction withX-Gal(#R0941) to determine thelacphenotype in blue/white colony screening.Applications• Blue/white colony screening to distinguish recombinant (white) from non-recombinant (blue) colonies (seeReference 1)• Induction of gene expression under control of thelacpromoterNote• Preparation of a 100 mM (23.83 mg/mL) stock solution in water is recommended• For blue/white colony screening use 0.1 mM final IPTG concentration in LB (Luria Broth) media• IPTG, dioxane-free, can be stored at +4°C
QQ:1749072012
