CD223 (LAG-3) Monoclonal Antibody (eBioC9B7W (C9B7W)), Functional Grade, eBioscience™/CD223 (LAG-3) 单抗 (eBioC9B7W (C9B7W)),功能抗体, eBioscience™
货号：16-2231-85, 16-2231-050
规格：500 µg,50 mg
价格：3618,124177
产品类型：流式抗体
品牌：eBioscience
抗原：CD223
物种：小鼠
宿主：大鼠
抗体亚型：IgG1, kappa
克隆号：eBioC9B7W (C9B7W)
荧光染料：其它
_ueditor_page_break_tag_
类型: | 一抗(功能抗体） | 同型对照： | Rat IgG1 kappa Isotype Control (eBRG1), Functional Grade, eBioscience™ |
浓度： | 1 mg/mL | 用法： | 0.5 µg/test（Flow） |
产品详细信息Description: The eBioC9B7W monoclonal antibody recognizes mouse CD223 (LAG-3, LAG3) protein expressed by activated alpha/beta-TCR bearing T cells, a subset of gamma/delta-TCR bearing T cells and a subset of NK cells. CD223 is a 70 kDa type I transmembrane protein with a structure that is similar to CD4. However, a soluble form of human CD223 has been detected by ELISA in human serum, and data suggest that mouse CD223 is proteolytically cleaved in the D4 domain. This results in a 54 kDa fragmentcontaining all the extracellular domains, and a 16 kDa fragment containing the intracellular and transmembrane domains. The 54 kDa can remain membrane-associated or be released as soluble CD223.CD223 binds to MHC class II with higher affinity than CD4, and it is thought that this interaction is involved in the negative regulation of T-cell activation and homeostatic proliferation. Furthermore, CD223 is expressed by CD4+CD25+ regulatory T cells, and it has been suggested that CD223 may be involved in their regulatory function.Applications Reported: This eBioC9B7W (C9B7W) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and ELISA. The functional grade eBioC9B7W has been reported for the in vitro and in vivo blocking of CD223 function.Applications Tested: This eBioC9B7W (C9B7W) antibody has been tested by flow cytometric analysis of anti-CD3 and anti-CD28-activated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息LAG-3 is a 70-kDa surface glycoprotein belonging to the Ig superfamily with homology to CD4. LAG-3 binds to MHC class II with higher affinity than CD4 and is thought to be involved in the negative regulation of T cell activation and homeostatic proliferation. Surface expression of LAG-3 has been reported on activated T cells (including regulatory T cells) and NK cells. CD8+ T cells usually express LAG-3 at significantly higher levels than CD4+ T cells. Coexpression of LAG-3 and CD49b has been proposed to identify human and mouse Type 1 regulatory T cells (Tr1 cells).仅用于科研。不用于诊断过程。未经明确授权不得转售。
数据 |
| CD223 (LAG-3) Antibody (16-2231-85) in FlowStaining of 3-day Anti-Mouse CD3 and anti-CD28-stimulated C57Bl/6 splenocytes with 0.25 µg of Rat IgG1 Isotype Control Purified (Product # 14-4301-82) (open histogram) or 0.25 µg of Anti-Mouse CD223 (LAG-3) Purified (filled histogram) followed by Anti-Rat IgG Biotin (Product # 13-4813-85) and Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis. |
推荐产品： |
| 二抗荧光二抗免疫组化一抗标签抗体 |
参考文献： |
| 流式1.Journal of immunology (Baltimore, Md. : 1950)Resident T Cells Are Unable To Control Herpes Simplex Virus-1 Activity in the Brain Ependymal Region during Latency."Published figure using CD223 (LAG-3) monoclonal antibody (Product # 16-2231-85) in Flow Cytometry"2.Nature communicationsRational combination of oncolytic vaccinia virus and PD-L1 blockade works synergistically to enhance therapeutic efficacy."Published figure using CD223 (LAG-3) monoclonal antibody (Product # 16-2231-85) in Flow Cytometry"3.Journal of immunology (Baltimore, Md. : 1950)Dendritic Cell-Based Vaccines that Utilize Myeloid Rather than Plasmacytoid Cells Offer a Superior Survival Advantage in Malignant Glioma."16-2231 was used in Flow cytometry/Cell sorting to compare the ability of myeloid and plasmacytoid dendritic cells to generate effective antiglioma immunity."AuthorsDey M,Chang AL,Miska J,Wainwright DA,Ahmed AU,Balyasnikova IV,Pytel P,Han Y,Tobias A,Zhang L,Qiao J,Lesniak MS4.BloodLymphatic endothelial cells induce tolerance via PD-L1 and lack of costimulation leading to high-level PD-1 expression on CD8 T cells."16-2231 was used in Flow cytometry/Cell sorting to demonstrate that LECs mediate deletion only via programmed cell death-1 (PD-1) ligand 1."AuthorsTewalt EF,Cohen JN,Rouhani SJ,Guidi CJ,Qiao H,Fahl SP,Conaway MR,Bender TP,Tung KS,Vella AT,Adler AJ,Chen L,Engelhard VH中和5.Journal of leukocyte biologyCD70 expression by dendritic cells plays a critical role in the immunogenicity of CD40-independent, CD4+ T cell-dependent, licensed CD8+ T cell responses."16-2231 was used in Blocking experiments to explain the capacity of CD40-deficient mice to mount CD8(+) T cell responses, which may provide targets for immunotherapy."AuthorsVan Deusen KE,Rajapakse R,Bullock TN |
QQ:1749072012