ViewRNA Tissue Assay Blue Module
货号：19932,19943
规格：24 assays,96 assays
价格：7007,17719
产品类型：核酸标记
品牌：Thermo Fisher
| This VewRNA Tissue Assay Blue Module is designed for use with the ViewRNA Tissue Assay Core Kit. The Core Kit, when combined with a ViewRNA Tissue probe set (purchased separately), enables the detection of a single mRNA target in tissue sections using the Fast Red substrate. The ViewRNA Tissue Assay Blue Module enables the detection of a second mRNA target using the Fast Blue substrate and must be used in combination with the Core Kit and two probes sets in order to run a 2-plex assay. Each kit contains sufficient reagents to process a minimum of six slides at a time. ViewRNA TYPE 1 probe sets are for use with the Fast Red substrate (Core Kit), while ViewRNA TYPE 6 probe sets are for use with the Fast Blue substrate (Blue Module).ViewRNA Tissue assays are RNA in situ hybridization (ISH) assays for the reliable detection of one or two mRNA targets within tissue sections. Visualize any gene in any tissue with single-copy sensitivity and no radioactivity. And in case your needs are not met by one of our 6500 catalog probe sets, we have decades of bioinformatics expertise and will design custom probe sets to any mRNA target of interest, free of charge.With ViewRNA Tissue assays, you can achieve:• Robust, single mRNA molecule detection in tissue sections• Accuracy and specificity inherent to branched DNA amplification technologies• Simultaneous detection of two RNA targetsThe ViewRNA Tissue Assay is compatible with formalin-fixed, paraffin-embedded (FFPE) tissue or OCT (optimal cutting temperature) embedded frozen tissue sections. Whereas traditional ISH technologies often amplify both signal and background, ViewRNA assays use branched DNA (bDNA) technology to amplify target-specific signal and achieve superior signal-to-noise ratios. |
| 数据 |
RNA-ISH of rat kidney using ViewRNA Assay Core KitRat kidney (FFPE) tissue was processed and probed with Synpo Type 1 Red, Spp1 Type 6 Blue using ViewRNA probe sets. These probe sets were detected using ViewRNA ISH Tissue Assay. RNA-ISH of human prostate using ViewRNA Assay Core KitHuman prostate (FFPE) tissue was processed and probed with KLK3 Type 1 Red, GAPDH Type 6 Blue using ViewRNA probe sets. These probe sets were detected using ViewRNA ISH Tissue Assay. RNA-ISH of mouse pancreas using ViewRNA Assay Core KitMouse pancreas (FFPE) tissue was processed and insulin and glucagon mRNA labeled using ViewRNA probe sets. These probe sets were detected using the ViewRNA ISH Tissue Assay, using ViewRNA Fast Red for insulin and ViewRNA Fast Blue for glucagon. Bright field (left) and fluorescent (middle) images were taken using the EVOS Imaging System. In the brightfield image ViewRNA Fast Red fluorescence is red, and ViewRNA Fast Blue is blue. In the fluorescent image, ViewRNA Fast Red fluorescence is pseudo-colored with red, and ViewRNA Fast Blue is pseudo-colored with green. A superimposed image of brightfield and fluorescence (right) demonstrates the flexibility of detection between brightfield and fluorescence. |
技术参数 组成成分 • 45 µL Label Probe 6-AP (2°C to 8°C)• 420 µL Blue Reagent 1°C(2 to 8°C)• 420 µL Blue Reagent 2°C(2 to 8°C)• 420 µL Blue Reagent 3°C(2 to 8°C)• 20 mL Blue Buffer (2°C to 8°C)• 10 mL AP Stop QT (2°C to 8°C)
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