Ly-108 Monoclonal Antibody (eBio13G3-19D (13G3-19D)), Functional Grade, eBioscience™/Ly-108单克隆(eBio13G3-19D (13G3-19D)), 功能抗体, eBioscience™
货号：16-1508-82
规格：100 µg
价格：3618
产品类型：流式抗体
品牌：eBioscience
抗原：Ly-108
物种：小鼠
宿主：小鼠
抗体亚型：IgG2a, kappa
克隆号：eBio13G3-19D (13G3-19D)
荧光染料：其它
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类型: | 一抗(功能抗体） | 同型对照： | Mouse IgG2a kappa Isotype Control (eBM2a), Functional Grade, eBioscience™ |
浓度： | 1 mg/mL | 用法： | 0.5 µg/test（Flow）;Assay-Dependent（IP）;Assay-Dependent（功能检测）;Assay-Dependent（WB） |
产品详细信息Description: The eBio13G3-19D monoclonal antibody reacts with mouse Ly108, the mouse homologue of human SLAMF6 (signaling lymphocyte activation molecule family member 6). Mouse Ly108 is also known as NTB-A (NK cell, T cell, B cell antigen), and is expressed on NK, T cells, and B cells. Engagement of the Ly108 receptor enhances NK cell activity and expression of IFN-gamma, and contributes to the inability of NK cells to kill Epstein Barr virus-infected B cells in X-linked lymphoproliferative disease. CD4+ T cells from Ly108 knock-out mice exhibit diminished ability to produce IL-4 and defective neutrophil functions.Staining has not revealed any strain differences between C57BL/6 and BALB/c mice. Other strains have not been tested.Applications Reported: This eBio13G3-19D (13G3-19D) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunoblotting (WB).Applications Tested: This eBio13G3-19D (13G3-19D) antibody has been tested by flow cytometric analysis of mouse splenocytes and thymocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息Ly108 is the mouse homologue of human SLAMF6 (signaling lymphocyte activation molecule family member 6). Mouse Ly108 is expressed on NK, B cells, and T cells. Activation of Ly108 receptor enhances NK cell activity and IFN-gamma expression.仅用于科研。不用于诊断过程。
数据 |
| Ly-108 Antibody (16-1508-82) in FlowStaining of BALB/c splenocytes with 0.25 µg of Mouse IgG2a kappa Isotype Control Purified (Product # 14-4724-82) (open histogram) or 0.25 µg of Anti-Mouse Ly-108 Purified (filled histogram) followed by Anti-Mouse IgG2a Biotin and Streptavidin PE (Product # 12-4317-87). Cells in the lymphocyte gate were used for analysis. |
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参考文献： |
| 阻断1.Journal of immunology (Baltimore, Md. : 1950)Differential Role of Hematopoietic and Nonhematopoietic Cell Types in the Regulation of NK Cell Tolerance and Responsiveness."16150882 was used in blocking or activating experiment to study the role of MCH I in natural killer cell tolerance and hyporesponsiveness"AuthorsShifrin NT,Kissiov DU,Ardolino M,Joncker NT,Raulet DH |
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