CD147 Monoclonal Antibody (RL73), Functional Grade, eBioscience™/CD147 单克隆 (RL73), 功能抗体, eBioscience™

货号：16-1471-82,16-1471-38,16-1471-025,16-1471-050

规格：100 µg,5 mg,25 mg,50 mg

价格：1992,24089,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：CD147

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：RL73

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	0.5 µg/test（Flow）;Assay-Dependent（对照）;Assay-Dependent（功能检测）；Assay-Dependent（IP）

产品详细信息Description: The RL73 monoclonal antibody reacts with mouse CD147, also known as Basigin. CD147 is expressed by a majority of thymocytes, activated peripheral T cells, erythrocytes and erythroblasts. It has been reported that CD147 expression correlates with cycling of immature thymocytes even in the absence of TCRbeta selection and that ligation of this molecule on immature fetal thymocytes inhibits their further development into mature T cells.Applications Reported: This RL73 antibodyhas been reported for use in flow cytometric analysis, and immunoprecipitation.Applications Tested: This RL73 antibody has been tested by flow cytometric analysis of mouse thymic cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息CD147 (basigin, neurothelin, OX-47, 5A11, CE9, M6, EMMPRIN, extracellular matrix metalloproteinase inducer, TCSF, tumour cell-derived collagenase-stimulatory factor) is a 50-60 kDa glycoprotein with multiple glycosylated forms. CD147 is a plasma membrane protein that is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. CD147 is also a member of the immunoglobulin superfamily. The highest level of CD147 expression is on metabolically active cells,such as lymphoblasts, inflammatory cells, brown adipocytes and malignant tumor cells. CD147 has multiple functions, including facilitating of cell surface expression of monocarboxylate transporter proteins and extracellular matrix metalloproteinases, regulation of integrin functions, it plays roles in cell development and activation, fetal development or retinal function.仅用于科研。不用于诊断过程。

数据

CD147 Antibody (16-1471-82) in FlowC57BL/6 thymocytes were stained with CD3 Monoclonal Antibody, APC (Product # 17-0031-82) and 0.25 µg Rat IgG2a kappa Isotype Control (Product # 14-4321-82) (left) or 0.25 µg CD147 Monoclonal Antibody, Functional Grade (right) followed by IgG Polyclonal Antibody, PE (Product # 12-4817-82). Total cells were used for analysis.

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参考文献：

流式，IF1.Journal of neuroinflammationA novel anti-EMMPRIN function-blocking antibody reduces T cell proliferation and neurotoxicity: relevance to multiple sclerosis."Published figure using CD147 monoclonal antibody (Product # 16-1471-82) in Flow Cytometry"功能检测2.Molecular medicine reportsCD147 blockade as a potential and novel treatment of graft rejection."16-1471 was used in a Functional assay to study the effects of CD147 blockade in vitro and in vivo and its use as an immunosuppressant drug to treat graft rejection."AuthorsLuan J,Zhao Y,Zhang Y,Miao J,Li J,Chen ZN,Zhu P

技术参数

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