CD317 (BST2, PDCA-1) Monoclonal Antibody (eBio927), Functional Grade, eBioscience™/CD317 (BST2, PDCA-1) 单克隆 (eBio927), 功能抗体, eBioscience™

货号：16-3172-85,16-3172-38,16-3172-025,16-3172-050

规格：500 µg,5 mg,25 mg,50 mg

价格：4085,24089,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：CD317

物种：小鼠

宿主：大鼠

抗体亚型：IgG2b, kappa

克隆号：eBio927

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Rat IgG2b kappa Isotype Control (eB149/10H5), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	0.25 µg/test（Flow）;Assay-Dependent（功能检测）

产品详细信息Description: The eBio927 monoclonal antibody reacts with PDCA-1 (BST2, CD317), a specific marker of plasmacytoid dendritic cells (pDC), also known as type I IFN-producing cells (IPC) in the naive mouse. Mouse IPCs are typically CD11c+, CD11b-, B220+, Ly-6C+, and CD62L+. PDCA-1 is predominantly expressed by IPCs in the naive mouse which represents a very minor population (<0.5%) of splenocytes. It is upregulated on numerous cell types following stimulation which triggers an IFN response. PDCA-1 cycles between cell surface and intracellular compartments and may function to regulate trafficking of secreted cytokines. PDCA-1 (BST2) is the protein recognized by antibody 120G8.The eBio927 monoclonal antibody has also been shown to have functional activity. The epitope recognized by eBio927 is distinct from eBio129c; thus, the antibodies can be used to costain, purify and identify pDCs.Applications Reported: This eBio927 antibody has been reported for use in flow cytometric analysis and for in vivo depletion studies as well as in vitro crosslinking studies.Applications Tested: This eBio927 antibody has been tested by flow cytometric analysis of BST-2 transfected cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息BST2 (CD317, HM1.24 antigen, DAMP-2, tetherin) is an integral membrane protein that is involved in the development and growth of B-cells. The surface expression of BST2 on fibroblast cell lines facilitated the stromal cell-dependent growth of DW34, a pre-B-cell line. BST2 is highly expressed during B-cell development, from pro-B precursors to plasma cells, T-cells, monocytes, NK cells and DCs (at protein level), and myeloma cells. Research studies have shown that BST2 also functions as an inhibitor of retrovirus release from human cells whose activity is antagonized by the HIV-1 accessory protein, Vpu. While BST2 causes retention of virions on cell surfaces or endocytosis into BST2-positive compartments, its depletion abolished the viral requirement for Vpu for virus release, an activity that may represent a potential therapeutic strategy for the treatment of HIV/AIDS. Another disease associated with BST2 dysfunction is stomatitis.仅用于科研。不用于诊断过程。

数据

CD317 (BST2, PDCA-1) Antibody (16-3172-85) in FlowStaining of BST2 transfected cells with 0.125 µg of Rat IgG2b Isotype Control Functional Grade Purified (Product # 16-4031-81) (open) or 0.125 µg of Anti-Mouse CD317 (BSTA, PDCA-1) Functional Grade Purified (filled) followed byAnti-Rat IgG PE (Product # 12-4822).Total viable cells were used for analysis.

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参考文献：

流式1.Scientific reportsOASL1 deficiency promotes antiviral protection against genital herpes simplex virus type 2 infection by enhancing type I interferon production."16-3172 was used in Flow cytometry/Cell sorting to investigate the role of 2'-5' oligoadenylate synthetase-like 1 (OASL1) during localised viral infection in mice."AuthorsOh JE,Lee MS,Kim YJ,Lee HK2.Proceedings of the National Academy of Sciences of the United States of AmericaDeficiency of transcription factor RelB perturbs myeloid and DC development by hematopoietic-extrinsic mechanisms."16-3172 was used in Flow cytometry/Cell sorting to suggest that there is an unrecognized but critical role for RelB within the nonhematopoietic niche that controls normal myelopoiesis."AuthorsBriseño CG,Gargaro M,Durai V,Davidson JT,Theisen DJ,Anderson DA,Novack DV,Murphy TL,Murphy KM3.Arthritis and rheumatology (Hoboken, N.J.)Bcl-2 antagonists kill plasmacytoid dendritic cells from lupus-prone mice and dampen interferon-α production."16-3172 was used in Flow cytometry/Cell sorting to evaluate the impact of Bcl-2 antagonists and glucocorticoids on plasmacytoid dendritic cells in vitro and in vivo."AuthorsZhan Y,Carrington EM,Ko HJ,Vikstrom IB,Oon S,Zhang JG,Vremec D,Brady JL,Bouillet P,Wu L,Huang DC,Wicks IP,Morand EF,Strasser A,Lew AMFN4.Journal of immunology (Baltimore, Md. : 1950)Peptidoglycan recognition protein 1 enhances experimental asthma by promoting Th2 and Th17 and limiting regulatory T cell and plasmacytoid dendritic cell responses."16-3172 was used in Functional assays to demonstrate the proinflammatory effect of peptidoglycan recognition protein (Pglyrp)1 during the development of allergic asthma."AuthorsPark SY,Jing X,Gupta D,Dziarski R

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