Ly-6G/Ly-6C Monoclonal Antibody (RB6-8C5), Functional Grade, eBioscience™/Ly-6G/Ly-6C 单克隆 (RB6-8C5), 功能抗体, eBioscience™

货号：16-5931-82,16-5931-85,16-5931-86,16-5931-38,16-5931-95

规格：100 µg,500 µg,1 mg,5 mg,10 mg

价格：1781,2980,5606,24089,29037

产品类型：流式抗体

品牌：eBioscience

抗原：Ly-6G/Ly-6C

物种：小鼠

宿主：大鼠

抗体亚型：IgG2b, kappa

克隆号：RB6-8C5

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Rat IgG2b kappa Isotype Control (eB149/10H5), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	0.5 µg/test（Flow）;Assay-Dependent（功能检测）

产品详细信息Description: The RB6-8C5 monoclonal antibody reacts with mouse Ly-6G, a 21-25 kDa protein also known as the myeloid differentiation antigen Gr-1. A GPI-linked protein, Gr-1 is expressed by the myeloid lineage in a developmentally regulated manner in the bone marrow. While monocytes only express Gr-1 transiently during their bone marrow development, the expression of Gr-1 on bone marrow granulocytes as well as on peripheral neutrophils is a good marker for these populations.eBioscience testing indicates that in the bone marrow and lysed whole blood, the antibody clone RB6-8C5 also stains cells that express the highest levels of Ly6c (as defined by staining with antibody clone HK1.4). It is recommended that 1A8-Ly6G (cat. 9668) be used when looking at Ly-6G specific targets.Applications Reported: The RB6-8C5 antibody has been reported for use in flow cytometric analysis. It has also been reported in cell depletion.Applications Tested: The RB6-8C5 antibody has been tested by flow cytometric analysis of mouse bone marrow cells and splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息Granulocytes are a category of white blood cells characterised by the presence of granules in their cytoplasm. They are also called polymorphonuclear leukocytes (PMN or PML) because of the varying shapes of the nucleus, which is usually lobed into three segments. In common parlance, the term polymorphonuclear leukocyte often refers specifically to neutrophil granulocytes, the most abundant of the granulocytes. Granulocytes or PMN are released from the bone marrow by the regulatory complement proteins.仅用于科研。不用于诊断过程。

数据

Ly-6G/Ly-6C Antibody (16-5931-82) in FlowStaining of mouse bone marrow with Anti-Human/Mouse CD45R (B220) PE (Product # 12-0452-82) and Anti-Mouse Ly-6G (Gr-1) FITC.Total viable cells were used for analysis.

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参考文献：

流式1.Cell discoveryAntiviral effects of ferric ammonium citrate."Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 16-5931-82) in Flow Cytometry"IHC2.JCI insightAnalysis of leukocyte transepithelial migration using an in vivo murine colonic loop model."Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 16-5931-82) in Immunofluorescence"IHC(F)3.The Journal of experimental medicineChemokines control naive CD8+ T cell selection of optimal lymph node antigen presenting cells."16-5931 was used in Flow cytometry/Cell sorting to demonstrate the ability of chemokines to assist antiviral CD8(+) T cells in distinguishing between antigen presenting cells."AuthorsHickman HD,Li L,Reynoso GV,Rubin EJ,Skon CN,Mays JW,Gibbs J,Schwartz O,Bennink JR,Yewdell JWIF4.PloS oneStroke Damage Is Exacerbated by Nano-Size Particulate Matter in a Mouse Model."Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 16-5931-82) in Immunofluorescence"中和5.Antioxidants and redox signalingRedox Regulation of Pro-IL-1β Processing May Contribute to the Increased Severity of Serum-Induced Arthritis in NOX2-Deficient Mice."16-5931 was used in Neutralization experiments to investigate how ROS act as a negative feedback to constrain IL-1β-mediated inflammation, accounting for the more severe arthritis in the absence of NOX2."AuthorsHuang YF,Lo PC,Yen CL,Nigrovic PA,Chao WC,Wang WZ,Hsu GC,Tsai YS,Shieh CC6. Shock (Augusta, Ga.)Mechanism of neutrophil recruitment to the lung after pulmonary contusion."16-5931 was used in Blocking experiments to investigate whether the innate immune and inflammatory activation of neutrophils figures prominently in the response to lung injury."AuthorsHoth JJ,Wells JD,Hiltbold EM,McCall CE,Yoza BK功能检测7.Infection and immunityNADPH oxidase promotes neutrophil extracellular trap formation in pulmonary aspergillosis."16-5931 was used in Functional assays to show that NADPH oxidase is required for pulmonary clearance of Aspergillus hyphae and generation of neutrophil extracellular traps in vivo."AuthorsRöhm M,Grimm MJ,D'Auria AC,Almyroudis NG,Segal BH,Urban CF8.AnesthesiologyRoles of Gr-1+ leukocytes in postincisional nociceptive sensitization and inflammation."16-5931 was used in Functional assays to identify the contribution of infiltrating neutrophils to incisional pain and inflammation."AuthorsSahbaie P,Li X,Shi X,Clark JD

技术参数

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