CD4 Monoclonal Antibody (RPA-T4), PE
货号：12-0049-80
规格：25 μg
价格：1029
产品类型：流式抗体
品牌：Thermo Fisher
物种：人
宿主：小鼠
抗体亚型：IgG1, kappa
荧光染料：PE
| 抗体类型 | 单抗 | 同型对照： | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE |
| 浓度： | 0.2 mg/mL | 用法： | 1 μg/test (Flow)； |
| 产品详细信息Description: The RPA-T4 monoclonal antibody reacts with human CD4, a 59 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells (T-helper cells) and in low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). RPA-T4 blocks HIV binding and mixed lymphocyte reaction. The RPA-T4 antibody recognizes a different epitope than the OKT4 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes.Applications Reported: The RPA-T4 antibody has been reported for use in flow cytometric analysis.Applications Tested: The FITC conjugated RPA-T4 has been tested by flow cytometric analysis and can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017]. |
| 数据 |
| CD4 Antibody (12-0049-80)Cancer immunology, immunotherapy : CII 2020 -Fig. 5 Characterization of generated T cells against LDHC-derived peptides. Multi-parameter flow cytometry was conducted to assess the frequency of CD4 + and CD8 + central memory (T CM ), effector memory (T EM ), naive (T N ), and effector (T E ) T cells. a Frequency of immune cell subsets among T cell responses after in vitro stimulation with peptides or control (no peptides). Representative density plots show an increase in CD4 + and CD8 + T EM cells of donor D03 after stimulation with PP4 as compared to control (no peptides)-stimulated T cells. b Frequency of immune cell subsets among T cell responses after priming with autologous pulsed-DCs. Representative density plots show an increase in CD4 + and CD8 + T EM s of donor D09 after stimulation with P11- and P73-pulsed DCs as compared to the cell subpopulations of T cells primed by control (no peptides)-pulsed DCs |
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| 参考文献： |
| 1.Frontiers in immunologyImpact of Enhancer of Zeste Homolog 2 on T Helper Cell-Mediated Allergic Rhinitis."Published figure using CD4 monoclonal antibody (Product # 12-0049-80) in Flow Cytometry"2.Molecular pharmaceuticsModulation of CD4+ T lymphocyte lineage outcomes with targeted, nanoparticle-mediated cytokine delivery."Published figure using CD4 monoclonal antibody (Product # 12-0049-80) in Flow Cytometry"3.Critical care (London, England)Upregulation of programmed death-1 on T cells and programmed death ligand-1 on monocytes in septic shock patients."12-0049 was used in Flow cytometry/Cell sorting to demonstrate that lymphocyte apoptosis is markedly increased in septic shock patients, and suggest an essential role for the PD-1/PD-L1 pathway in sepsis-induced immunosuppression."4.Acta Cardiologica SinicaThe Effects of High Mobility Group Box-1 Protein on Peripheral Treg/Th17 Balance in Patients with Atherosclerosis."Published figure using CD4 monoclonal antibody (Product # 12-0049-80) in Flow Cytometry"5.CellRAB11FIP5 Expression and Altered Natural Killer Cell Function Are Associated with Induction of HIV Broadly Neutralizing Antibody Responses."Published figure using CD4 monoclonal antibody (Product # 12-0049-80) in Flow Cytometry" |
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