FOXP3 Monoclonal Antibody (236A/E7), Alexa Fluor 488, eBioscience™

货号：53-4777-42,53-4777-41

规格：100 Tests,25 Tests

价格：3305,1577

产品类型：流式抗体

品牌：eBioscience

抗原：FOXP3

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：236A/E7

荧光染料：Alexa Fluor 488

类型:	一抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor 488, eBioscience™
浓度：	5 µL/Test	用法：	5 µL (0.5 µg)/test（Flow）

产品详细信息Description: The 236A/E7 antibody reacts with human Foxp3 protein also known as FORKHEAD BOX P3, SCURFIN, and JM2. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of Foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.Intracellular staining and flow cytometric analysis of freshly isolated human peripheral blood mononuclear cells (PBMCs) with the 236A/E7 antibody using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol reveals staining of the CD4+CD25bright population.The epitope from 236A/E7 is different from that of PCH101 (cat. 72-5776). This antibody has also been shown to recognize rhesus macaque, sooty mangabey and cynomolgus macaque.Applications Reported: This 236A/E7 antibody has been reported for use in intracellular staining followed by flow cytometric analysis and IHC.Applications Tested: This 236A/E7 antibody has been pre-titrated and tested using the Foxp3/Transctiption Factor Staining Buffer Set (cat. 00-5523) and protocol. Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells. Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice. In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome. Transcript variants of FOXP3 encoding different isoforms have been identified. In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.仅用于科研。不用于诊断过程。

数据

FOXP3 Antibody (53-4777-42)Intracellular staining of human peripheral blood cells. As expected based on known expression patterns, Foxp3 clone 236A/E7 stains CD4+CD25+ T cells and does not stain CD4- cells or CD4+CD25- T cells. Details: Normal human peripheral blood cells were surface stained with CD4 (clone OKT4) and CD25 (clone BC96) followed by intracellular staining with Foxp3 (clone 236A/E7) using the Foxp3/Transcription Factor Staining Buffer Set and protocol. Lymphocytes in the CD4- (left) and CD4+ (right) gates were used for analysis.FOXP3 Antibody (53-4777-42)Oncotarget 2017 -Figure 6 FoxP3 and Helios expression in CD4 + T cells PBMC from HD and PBC patients, NILs and TILs were stained for CD3 and CD4 antibodies followed by intracellular staining for FoxP3 and Helios. Live cells were first gated using Fixable Viability Dye 660. ( A ). Representative flow cytometric plots of FoxP3 staining from one cancer patient are shown. ( B ). Scatter plot showing the differences in frequencies of FoxP3 + Tregs between different samples. ( C ). IHC staining of FoxP3 + expression in one NT and TT samples. ( D ). Flow cytometric plots of FoxP3 and Helios co-expression in CD4 + T cells from different samples and whisker plots ( E ) showing differences in various FoxP3 and Helios-expressing Treg subsets. ( F ). Non-parametric Spearman and apos;s test showing correlations between FoxP3 and Helios expressions in NILs and TILs. ( G ). Pie charts show the relative percentages of different FoxP3 and Helios Treg subsets.

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参考文献

1.Experimental hematology and oncologyChanges in peripheral blood lymphocytes in polycythemia vera and essential thrombocythemia patients treated with pegylated-interferon alpha and correlation withJAK2V617Fallelic burden."Published figure using FOXP3 monoclonal antibody (Product # 53-4777-42) in Flow Cytometry"2.OncotargetHDAC inhibition potentiates immunotherapy in triple negative breast cancer."Published figure using FOXP3 monoclonal antibody (Product # 53-4777-42) in Flow Cytometry"3.ImmunityRegulatory T Cell Migration Is Dependent on Glucokinase-Mediated Glycolysis."Published figure using FOXP3 monoclonal antibody (Product # 53-4777-42) in Flow Cytometry"4.Nature communicationsFollicular regulatory T cells impair follicular T helper cells in HIV and SIV infection."Published figure using FOXP3 monoclonal antibody (Product # 53-4777-42) in Flow Cytometry"5.The Journal of infectious diseasesCD4(+) regulatory T cells in a cynomolgus macaque model of Mycobacterium tuberculosis infection."Published figure using FOXP3 monoclonal antibody (Product # 53-4777-42) in Immunofluorescence"

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