CD279 (PD-1) Monoclonal Antibody (MIH4), eFluor 450
货号：48-9969-42
规格：100 Tests
价格：2822
产品类型：流式抗体
品牌：Thermo Fisher
物种：人
宿主：小鼠
抗体亚型：IgG1, kappa
荧光染料：eFluor 450
| 抗体类型 | 单抗 | 同型对照： | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor 450 |
| 浓度： | 5 µL/Test | 用法： | 5 μL (0.5 μg)/test (Flow) |
| 产品详细信息Description: The MIH4 monoclonal antibody reacts with the human PD-1 (programmed death-1), a 55 kDa member of the immunoglobulin superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease. PD-1 is expressed predominantly on activated T and B lymphocytes. Two novel members of the B7 family have been identified as the PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC). Evidence reported to date suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. The MIH4 antibody recognizes a different epitope than antibody clones J105.Applications Reported: This MIH4 antibody has been reported for use in flow cytometric analysis.Applications Tested: This MIH4 antibody has been pre-diluted and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This may be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.eFluor® 450 is a replacement for Pacific Blue™. eFluor® 450 emits at 445 nm and is excited with the violet laser line (405 nm). Please make sure that your instrument is capable of detecting this fluorochrome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser靶标信息Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of theimmune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE. |
| 数据 |
| CD279 (PD-1) Antibody (48-9969-42) in FlowNormal human peripheral blood cells were unstimulated (left) or stimulated with PHA-L (Product # 00-4977-03) (right). Cells were then stained with Mouse IgG1 kappa Isotype Control, eFluor® 450 (Product # 48-4714-82) (blue histogram) or CD279 Monoclonal Antibody, eFluor® 450 (purple histogram). Total viable cells were used for analysis, as determined by 7-AAD (Product # 00-6993-50). |
| 推荐产品： |
| 二抗荧光二抗免疫组化一抗标签抗体 |
QQ:1749072012