CD8a Monoclonal Antibody (4SM15), Biotin
货号：13-0808-82
规格：100μg
价格：3512
产品类型：流式抗体
品牌：eBioscience
物种：小鼠
宿主：大鼠
抗体亚型：IgG2a, kappa
荧光染料：Biotin
| 抗体类型 | 一抗 | 同型对照： | Rat IgG2a kappa Isotype Control (eBR2a), Biotin |
| 浓度： | 0.5 mg/mL | 用法： | 0.25 μg/test (Flow)； |
| 产品详细信息Description: The 4SM15 monoclonal antibody reacts with the mouse CD8a molecule. CD8a is an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alpha alpha). A majority of thymocytes and a subpopulation of mature alpha beta TCR T cells express CD8 alpha beta while gamma delta TCR T cells, a subpopulation of intestinal intraepithelial lymphocytes (IELs) and dendritic cells express CD8 alpha alpha. CD8 bindsto MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells.Recent studies have shown the 4SM15 antibody recognizes and binds a non-CD8a epitope in neural tissue. The 4SM15 clone is not recommended for staining of mouse neural tissue. For additional information please contact our technical service (tech@eBioscience.com). For detection of mouse CD8a in FFPE mouse neural tissue please refer to alternate product Anti-Mouse CD8a Purified (cat. no 14-0195).The 4SM15 monoclonal antibody is not recommended for immunohistochemistry of frozen mouse tissue. For detection of mouse CD8a using immunohistochemistry of frozen tissue please refer to alternate product Anti-Mouse CD8a Purified (cat. no. 14-0081).Applications Reported: This 4SM15 antibody has been reported for use in flow cytometric analysis, microscopy, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.Applications Tested: This 4SM15 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded mouse tissue using low or high pH antigen retrieval and can be used at less than or equal to 10 µg/mL. The 4SM15 antibody has been tested by flow cytometric analysis of mouse splenocytes and can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD8 molecule is composed of two chains termed alpha and beta. CD8 is found on a T cell subset of normal cytotoxic / suppressor cells which make up approximately 20 to 35% of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer cells, 80% of thymocytes, on a subpopulation of 30% of peripheral blood null cells and 15 to 30% of bone marrow cells. |
| 数据 |
| CD8a Antibody (13-0808-82) in IHC (P)Immunohistochemistry of formalin-fixed paraffin embedded mouse spleen using 10 µg/mL Anti-Mouse CD8a Biotin, followed by Streptavidin HRP and DAB visualization. Nuclei are counterstained with hematoxylin. |
| 推荐产品： |
| 二抗荧光二抗免疫组化一抗标签抗体 |
| 参考文献： |
| 1.JCI insightPhysiologically activated mammary fibroblasts promote postpartum mammary cancer."Published figure using CD8a monoclonal antibody (Product # 13-0808-82) in Immunohistochemistry"2.Cancer cellCooperation between Constitutive and Inducible Chemokines Enables T Cell Engraftment and Immune Attack in Solid Tumors."Published figure using CD8a monoclonal antibody (Product # 13-0808-82) in Immunohistochemistry"3.Nature communicationsNano-enabled pancreas cancer immunotherapy using immunogenic cell death and reversing immunosuppression."Published figure using CD8a monoclonal antibody (Product # 13-0808-82) in Immunohistochemistry"4.Cell reportsAn Immunocompetent Mouse Model of HPV16(+) Head and Neck Squamous Cell Carcinoma."Published figure using CD8a monoclonal antibody (Product # 13-0808-82) in Immunohistochemistry"5.Nature communicationsNano-enabled pancreas cancer immunotherapy using immunogenic cell death and reversing immunosuppression."Published figure using CD8a monoclonal antibody (Product # 13-0808-82) in Immunohistochemistry" |
©
Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：
https://www.biorod.cn/thread-27547.htm
QQ:1749072012