| 产品介绍 | | |
| Human anti Human vimentin antibodyrecognizes human vimentin, a class III intermediate filament of ~ 54kDa. Vimentin is preferentially expressed in mesenchymal tissues, and within the immune system is retained throughout T-cell development, with decreasing expression in B lymphocytes with maturation.In lymphatic tissues vimentin expression is seen in marginal zone areas, but not in follicle centers. A lack of vimentin expression in B cell lymphoma is indicative of follicular center origin.Human anti Human vimentin antibody recognizes vimentin as a band of ~54 kDa in a number of human cell line lysates by western blotting under reducing conditions. |
| 产品详情 | | |
| Target Species | Human |
| Product Form | A bivalent human recombinant Fab selected from the HuCAL GOLD phage display library, expressed inE. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This product is supplied as a liquid. |
| Preparation | Recombinant human monoclonal antibody purified by metal chelate affinity chromatography |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3) |
| Immunogen | Formalin fixed, paraffin-embedded human lymphoma tissue |
| Approx. Protein Concentrations | F(ab) concentration 0.5 mg/ml |
| Acknowledgements | PrecisionAb is a trademark of Bio-Rad Laboratories.This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.His-tag is a registered trademark of EMD Biosciences. |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| ELISA | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Flow CytometryUse 10ul of the suggested working dilution to label 106cells or 100ul human whole blood. |
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| 文献 |
| 1.Jarutat, T.et al.(2007) Selection of vimentin-specific antibodies from the HuCAL phage display library by subtractive panning on formalin-fixed, paraffin-embedded tissue.Biol Chem. 388 (6): 651-8. |
QQ:1749072012