| 产品介绍 | | |
| Rat anti MBP antibody, clone 14recognizes myelin basic protein (MBP), a component of myelin that is beleived to play a role in the myelination of nerves in the central nervous system.Rat anti MBP antibody, clone 14 has also been reported to work in western blotting (Relucioet al.2009). |
| 产品详情 | | |
| Target Species | Bovine |
| Product Form | Tissue Culture Supernatant - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant. |
| Buffer Solution | Phosphate buffered saline |
| Immunogen | Bovine myelin basic protein |
| Fusion Partners | Spleen cells from immunised outbred rats were fused with cells of the mouse NS0 myeloma cell line. |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
| Preservative Stabilisers | <0.1% Sodium Azide (NaN3) |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| ELISA | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 推荐产品 |
| Description | Product Code | Applications | Pack Size |
| Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) | STAR117A | F | 0.5mg |
| 文献 |
| 1.Groome, N.P.et al.(1986) Region-specific immunoassays for human myelin basic protein.J Neuroimmunol. 12 (4): 253-64.2.Glynn, P.et al.(1987) Basic protein dissociating from myelin membranes at physiological ionic strength and pH is cleaved into three major fragments.J Neurochem. 48 (3): 752-9.3.Groome, N.et al.(1988) New monoclonal antibodies reactive with defined sequential epitopes in human myelin basic protein.J Neuroimmunol. 19 (4): 305-15.4.Matsuo, A.et al.(1997) Unmasking of an unusual myelin basic protein epitope during the process of myelin degeneration in humans: a potential mechanism for the generation of autoantigens.Am J Pathol. 150: 1253-66. |
QQ:1749072012