Mouse anti Human CD48 antibody, clone MEM-102
货号：MCA1053F
规格：0.1 mg
价格：2865
产品类型：流式抗体
品牌：Bio-Rad
抗原：CD48
物种：人
宿主：小鼠
抗体亚型：IgG1
克隆号：MEM-102
荧光染料：FITC
| 产品介绍 | | |
| Mouse anti Human CD48 antibody, clone MEM-102recognizes human CD48, also known as B-lymphocyte activation marker BLAST-1, BCM1 surface antigen, Leukocyte antigen MEM-102, SLAM family member 2 or Signaling lymphocytic activation molecule 2. CD 48, as a mature molecule is a 193 amino acid, ~45 kDa cell membrane glycoprotein containing 2 Ig-like domains.CD48 acts as a counter-receptor for CD2 (Katoet al.1992) and is involved in immune regulation and tolerance (McArdelet al.2016)Mouse anti Human CD48 antibody, clone MEM-102 is useful for the visualisation of CD48 expressing cells by immunofluorescence (Enose-Akahataet al.2009) and flow cytometry and may be used to differentiate haemapoietic tumors, sarcomas and melanomas. |
| 产品详情 | | |
| Target Species | Human |
| Species Cross-Reactivity | Target Species | Cross Reactivity |
| Rhesus Monkey | √ |
| Product Form | Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein A |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin |
| Immunogen | Con A activated canine peripheral blood cells. |
| Approx. Protein Concentrations | IgG concentration 0.1 mg/ml |
| Fusion Partners | Spleen cells from immunised DA rats were fused with cells of the rat Y3/Ag1.2.3 myeloma cell line. |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| FITC | 490 | 525 |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | | Neat |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Flow CytometryUse 10ul of the suggested working dilution to label 106cells or 100ul human whole blood. |
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| 文献 |
| 1.Cobbold, S. & Metcalfe, S. (1994) Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW).Tissue Antigens. 43 (3): 137-54. |
产品应用 Flow
产品类型 单克隆抗体
推荐用于 Flow
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