Sheep anti Human erythropoietin receptor antibody
货号：AHP1825
规格：0.1 mg
价格：2465
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原： erythropoietin receptor
物种：人
宿主：Sheep
抗体亚型：IgG
荧光染料：Purified
| 产品介绍 | | |
| Sheep anti Human erythropoietin receptor antibodyrecognizes the human Erythropoietin Receptor (EPOR), a type I transmembrane protein and member of the type I cytokine receptor family, expressed by erythroid cells and erythroid progenitor cells, which acts as a receptor for erythropoietin (EPO) to mediate erythroblast proliferation and differentiation, and maintain progenitor survival.Binding of EPO to EPOR, results in the dimerization of the EPOR and the triggering of the JAK2/STAT5 signaling cascade, and in some cell types activation of STAT1 and STAT3. Several isoforms of EPOR exist: EPOR-F, EPOR-S and EPOR-T, and a functional EPOR is found on endothelial cells and cardiomyocytes within the cardiovascular system, suggesting a role in cardiac function.Sheep anti Human erythropoietin receptor antibody (AHP1825) blocks Iodine125labelled erythropoietin ligand binding using erythroleukemia cell line TF-1 with 3000 EPOR/cells (Harriset al.1992).Sheep anti Human erythropoietin receptor antibody is suitable for use in immunocytochemistry and for use in functional assays. Removal of sodium azide is recommended prior to use in functional assays. |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified IgG - liquid |
Antiserum Preparation | Antiserum to human EPOR was raised by repeated immunisation of sheep with highly purified antigen. Purified IgG was prepared by affinity chromatography. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | <0.1% Sodium Azide (NaN3) |
Approx. Protein Concentrations | IgG concentration 1.0mg/ml |
Immunogen | Partial cDNA of the extracellular domain of human EPOR expressed as a GST fusion protein inE. Coli. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| Store at +4℃.DO NOT FREEZE.This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | 50ng/ml | |
Immunoprecipitation | √ | 10ug/ml | |
Western Blotting | √ | 10ug/ml | |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- ELISA
- This product is suitable for use in indirect ELISA applications.
- Western Blotting
- AHP1825 detects a 49kDa band of EPOR-GST on SDS-PAGE.
|
| 二抗相关产品 |
Description | Product Code | Applications | Pack Size |
| Rabbit anti Sheep IgG (H/L):Biotin | 5184-2304 | C;E;F;WB | 1.5 ml |
| Donkey anti Sheep/Goat IgG:DyLight®488 | STAR88D488GA | F;IF | 0.1 mg |
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | C;E;P;WB | 1 ml |
| 文献 |
| 1. Harris, K.W.et al.(1992) Ligand binding properties of the human erythropoietin receptor extracellular domain expressed inEscherichia coli.J Biol Chem. 267 (21): 15205-9. |
技术参数 产品应用 ELISA;IP;WB
产品类型 Polyclonal Antibody
推荐用于 ELISA;IP;WB
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