Rabbit anti Human SMAD3 antibody
货号：AHP1465
规格：50 µg
价格：7718
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原： SMAD3
物种：人
宿主：兔
抗体亚型：IgG
荧光染料：Purified
| 产品介绍 | | |
| Rabbit anti Human SMAD3 antibodyrecognizes Mothers against decapentaplegic homolog 3 (SMAD3), a 50 kDa transcriptional modulator activated by TGF-beta and activin type 1 receptor kinase,SMAD3 functions in cell proliferation, differentiation and development. Upon stimulation, SMAD3 oligomerizes with SMAD4 and translocates into the nucleus where it binds to DNA and transcription factors. SMAD3 activity induces differentiation of selected neuronal subtypes at the expense of other subtypes.Defects in SMAD3 may be a cause of colorectal cancer (Sjöblomet al.2006). |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified IgG - liquid |
Antiserum Preparation | Antiserum to human SMAD3 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | <0.1% Sodium Azide (NaN3) |
Approx. Protein Concentrations | IgG concentration 1.0mg/ml |
Immunogen | Synthetic peptide corresponding to amino acids 417-425 of human SMAD3 protein. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | 1/25000 | 1/100000 |
Immunohistology - Paraffin1 | √ | | 2.5ug/ml |
Western Blotting | √ | 1/2000 | 1/20000 |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- Histology Positive Control Tissue
- Human skin
|
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技术参数 产品应用 ELISA;IHC(P)*;WB
产品类型 Polyclonal Antibody
推荐用于 ELISA;IHC(P)*;WB
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