产品介绍 |
| Rabbit anti West Nile Virus antibodyrecognizes the ~14 kDa core protein of West Nile Virus (WNV). WNV belongs to the Flaviviridae family which includes closely viruses such as Dengue fever, Yellow fever and Tick borne encephalitis.West Nile Virus particles consist of a dense core made up of the core/capsid protein encapsulating the RNA genome surrounded by a membrane envelope containing envelope and matrix proteins. Research indicates that the viral core protein contributes to the WNV-associated inflammation via apoptosis induced though the caspase-9 pathway.West Nile Virus is spread by mosquitos and is believed to have originated in the West Nile region of Africa but has now spread to Europe, Asia and America where it has become a significant disease affecting human and domestic animal health. |
产品详情 |
Target Species | Viral |
Product Form | Purified IgG - liquid |
AntiserumPreparation | Antiserum to human West Nile Virus core protein was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | 0.02% Sodium Azide (NaN3) |
Immunogen | A peptide corresponding to an 15 amino acid sequence from near the carboxy terminus of West Nile Virus core protein. |
Approx. Protein Concentrations | IgG concentration 1.0mg/ml |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
存储条件 |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
应用 |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | | 1ug/ml |
Immunofluorescence | √ | | 20ug/ml |
Western Blotting | √ | | 1ug/ml |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- Western Blotting
- AHP1666 detects a band of approximately 14kDa in Transfected HeLa cell lysate
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可用二抗 |
Description | Product Code | Applications | Pack Size |
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
相关产品 |
Description | Product Code | Applications | Pack Size |
TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
文献 |
| 1.Gould, L.H. & Fikrig, E. (2004) West Nile virus: a growing concern?J Clin Invest. 113 (8): 1102-7.2.Chu, J.J.et al.(2005) Inhibition of West Nile virus entry by using a recombinant domain III from the envelope glycoprotein.J Gen Virol. 86 (Pt 2): 405-12.3.Chu, J.J. & Ng, M.L. (2004) Interaction of West Nile virus with alpha v beta 3 integrin mediates virus entry into cells.J Biol Chem. 279 (52): 54533-41.4.Yang, J.S.et al.(2002) Induction of inflammation by West Nile virus capsid through the caspase-9 apoptotic pathway.Emerg Infect Dis. 8 (12): 1379-84. |
QQ:1749072012