| 产品介绍 | | |
| Goat anti Horse IgG (T) antibodyrecognizes equine IgG (T). No cross-reactivity with other equine immunoglobulin classes is seen in immuno-electrophoresis.Goat anti Horse IgG (T) antibody may cross react with IgG from other species. |
| 产品详情 | | |
Target Species | Horse |
Product Form | Purified IgG- liquid |
| Antiserum Preparation | Antisera to equine IgG (T) were raised by repeated immunisation of goat with highly purified antigen. Purified IgG prepared by affinity chromatography. |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide (NaN3) |
Immunogen | Purified equine IgG (T). |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom dateof despatch |
| 存储条件 | | |
| Store at +4oC. DO NOT FREEZE.This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | 1/100 | 1/1000 |
| Immunodiffusion | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. |
| 文献 |
| 1. Hooper-McGrevy, K.E.et al.(2003) Immunoglobulin G subisotype responses of pneumonic and healthy, exposed foals and adult horses toRhodococcus equivirulence-associated proteins.Clin Diagn Lab Immunol. 10 (3): 345-51.2. Jacks, S.et al.(2007) Experimental infection of neonatal foals withRhodococcus equitriggers adult-like gamma interferon induction.Clin Vaccine Immunol. 14: 669-77.3. Lewis, M.J.et al.(2007) The different effector function capabilities of the seven equine IgG subclasses have implications for vaccine strategies.Mol Immunol. 45: 818-27.4. Ryan, C. & Giguère, S. (2010) Equine neonates have attenuated humoral and cell-mediated immune responses to a killed adjuvanted vaccine compared to adult horses.Clin Vaccine Immunol. 17 (12): 1896-902.5. Cauchard Set al.(2014) Assessment of the safety and immunogenicity ofRhodococcus equi-secreted proteins combined with either a liquid nanoparticle (IMS 3012) or a polymeric (PET GEL A) water-based adjuvant in adult horses and foals--identification of promising new candidate antigens.Vet Immunol Immunopathol. 157 (3-4): 164-74.6. Meulenbroeks Cet al.(2015) Allergen-Specific Cytokine Polarization Protects Shetland Ponies againstCulicoides obsoletus-Induced Insect Bite Hypersensitivity.PLoS One. 10 (4): e0122090.7. Cauchard, S.et al.(2014) Assessment of the safety and immunogenicity ofRhodococcus equi-secreted proteins combined with either a liquid nanoparticle (IMS 3012) or a polymeric (PET GEL A) water-based adjuvant in adult horses and foals--identification of promising new candidate antigens.Vet Immunol Immunopathol. 157 (3-4): 164-74.8. Burk, S.V.et al.(2016) Equine antibody response to larvalParascaris equorumexcretory-secretory products.Vet Parasitol. 226: 83-7.9. Lightbody, K.L.et al.(2016) Validation of a novel saliva-based ELISA test for diagnosing tapeworm burden in horses.Vet Clin Pathol. 45 (2): 335-46. |
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