IL-18BP Human ELISA Kit/人IL-18BP ELISA试剂盒
货号：EHIL18BP,EHIL18BPX5,EHIL18BPX10
规格：96 tests,5X96 tests,10X96 tests
价格：7311,29891,52892
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 20 pg/mL |
| 检测范围 | 24.69-18,000 pg/mL |
样本类型/体积 | 上清液 100 μL 血清 33 μL 血浆 33 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr 45 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | <12% |
| 批内变异系数（CV） | <10% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | IL18BP |
| Protein aliases | IL-18BP, Tadekinig-alfa, Interleukin-18-binding protein, MC51L-53L-54L homolog gene product |
物种（Tested） | 人 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | IL18BP, IL18BPa |
| Gene ID | (Human) 10068 |
| Gene symbol | IL18BP |
| UniProt ID | (Human) O95998 |
| The Human Interleukin 18 Binding Protein (Hu IL-18BP) ELISA quantitates Hu IL-18BP in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-18BP.人白介素18结合蛋白（Hu IL-18BP）ELISA定量了人血清，血浆或细胞培养基中的人IL-18BP。 该检测试剂盒将专门识别天然和重组的人IL-18BP。Principle of the method 方法原理The Human IL-18BP solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人IL-18BP固相夹心ELISA（酶联免疫吸附测定）设计用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息IL-18 (also referred to as IL-1γ) has been shown to augment the secretion of IFN-γ from T lymphocytes and to increase NK cell activity in spleen cells. IL-18 exhibits 19% and 12% identity with IL-1α and IL-β, respectively, over the twelve β-strands of the β-trefoil fold domain, which is a signature feature of the IL-1 family. The unusual leader sequence of IL-18 may be analogous to the IL-1β pro-domain, which must be cleaved by the serine protease ICE for optimal secretion and biological activity. IL-18, which was originally described as IGIF (IFN-γ-inducing factor), IL-18 is induced in mouse liver subsequent to challenge with lipopolysaccharide (LPS). IL-18 binding protein (IL-18BP) functions as an inhibitor of the early Th1 response by binding to IL-18 and inhibiting IFN-γ production. IL-18BP is a member of the immunoglobulin superfamily and shares some homology to IL-1RII.IL-18（也称为IL-1γ）可增加T淋巴细胞中IFN-γ的分泌并增加脾细胞中NK细胞的活性。 IL-18在三叶折叠域的十二个β链上分别与IL-1α和IL-β表现出19％和12％的同一性，这是IL-1家族的标志性特征。 IL-18的异常前导序列可能类似于IL-1β前结构域，必须通过丝氨酸蛋白酶ICE对其进行切割才能获得最佳的分泌和生物学活性。 IL-18，最初被描述为IGIF（IFN-γ诱导因子），在用脂多糖（LPS）攻击后在小鼠肝脏中诱导IL-18。 IL-18结合蛋白（IL-18BP）通过与IL-18结合并抑制IFN-γ的产生而成为Th1早期反应的抑制剂。 IL-18BP是免疫球蛋白超家族的成员，与IL-1RII具有某些同源性。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
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